000			02902cam a2200349 a 4500
003			EG-GiCUC
005			20250223032745.0
008			210605s2021 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.12.02.Ph.D.2021.Ha.C
100	0		_aHadeer Hesham Abdelfattah Aly
245	1	0	_aCirculating MicroRNAs as a prognostic biomarker predicting clinical outcome in breast cancer / _cHadeer Hesham Abdelfattah Aly ; Supervised Hamed Abdelfatah Dabaon , Mohamed Aly Eldessouky , Neemat Mohamed Aly Kassem
246	1	5	_aالاحماض الميكرونووية ريبوزية الدوارة كعلامة بيولوجية نذيرة للتنبؤ بالنتيجة السريرية فى سرطان الثدى
260			_aCairo : _bHadeer Hesham Abdelfattah Aly , _c2021
300			_a112 P. : _bcharts ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry
520			_aBreast cancer is one of the most common cancers in women worldwide. Over 1.5 million women (25% of all women with cancer) are diagnosed with breast cancer every year throughout the world. Purpose & Methodology Quantitative assessment of plasma miRNA-34a & miRNA-125b expression was performed by qRT-PCR to predict outcome from neoadjuvant chemotherapy. Thirty nine newly diagnosed locally advanced breast cancer female patients with 10 age and sex matched healthy volunteers were included in the study. Results We performed ROC curve analysis to evaluate the diagnostic value for the miRNA-34a with AUCs = 0.995, cutoff point of 2.57 sensitivity 97.4%, specificity 100%, PPV 100%, NPV 83.3% and accuracy 97.7%.miRNA-125b had AUC = 0.68 and a cutoff point of 8.69 with sensitivity 66.7%, specificity 70.0%, PPV 90.6%, NPV 41.2% and accuracy 73.5%. miRNA-34a expression were significantly higher in breast cancer patients compared to controls with p value <0.001*. Also, miRNA-34a expression level was significantly higher in patients with progressive disease with P value =0.03*. However, miRNA-125b expression levels were insignificantly higher in responsive patients with p value = 0.2. Conclusion miRNAs are crucial candidates for novel molecular targeted therapies due to their capability to regulate numerous genes in molecular pathways. miRNA-34a can predict chemotherapeutic resistance associated with higher expression levels in non-responsive patients
530			_aIssued also as CD
653		4	_aBreast cancer
653		4	_aDNA technology
653		4	_aGene expression
700	0		_aHamed Abdelfatah Dabaon , _eSupervisor
700	0		_aMohamed Aly Eldessouky , _eSupervisor
700	0		_aNeemat Mohamed Aly Kassem , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c81177 _d81177