000			02795cam a2200349 a 4500
003			EG-GiCUC
005			20250223032747.0
008			210612s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.12.02.Ph.D.2021.Na.S
100	0		_aNada Sabry Ibraheem Abdelhady
245	1	0	_aSynthesis and biological evaluation of novel chalcones as anticancer agents / _cNada Sabry Ibraheem Abdelhady ; Supervised Ismail Abdelshafy Abdelhamid , Magda Fikry Mahmoud Mohamed , Salwa Mohamed Elhallouty
246	1	5	_aتشييد وتقييم بيولوجى لشالكونات جديده كمضادات للسرطان
260			_aCairo : _bNada Sabry Ibraheem Abdelhady , _c2021
300			_a144 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry
520			_aTwo novel chemotherapeutic chalcones were synthesized and their structures were confirmed by different spectral tools.Theoretical studies such as molecular modeling were done to detect the mechanism of action of these compounds. In vitro cytotoxicity showed a strong effect against all tested cell lines (MCF7, A459, HepG2, and HCT116), and low toxic effect against normal human melanocytes (HFB4).The lung carcinoma cell line was chosen for further molecular studies. Real-time PCR demonstrated that the two compounds upregulated gene expression of (BAX, p53, casp-3, casp-8, casp-9) genes and decreased the expression of anti-apoptotic genes bcl2, CDK4, and MMP1.Flow-cytometry indicated that cell cycle arrest of A459 was induced at the G2/M phase and the apoptotic percentage increased significantly compared to the control sample. Cytochrome c oxidase and VEGF enzyme activity were detected by ELISA assay. SEM tool was used to follow the morphological changes that occurred on the cell surface, cell granulation, and average roughness of the cell surface. The change in the number and morphology of mitochondria, cell shrinkage, increase in the number of cytoplasmic organelles, membrane blebbing, chromatin condensation, and apoptotic bodies were observed using TEM. The obtained data suggested that new chalcones exerted their pathways on lung carcinoma through induction of two pathways of apoptosis
530			_aIssued also as CD
653		4	_a[l, 2, 4]Triazolo[3,4-a]isoquinoline
653		4	_aChalcones
653		4	_aCytotoxicity
700	0		_aIsmail Abdelshafy Abdelhamid , _eSupervisor
700	0		_aMagda Fikry Mahmoud Mohamed , _eSupervisor
700	0		_aSalwa Mohamed Elhallouty , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c81253 _d81253