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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.12.21.M.Sc.2021.Al.A
100 0 _aAlaa Hussien Saleh Bashry
245 1 0 _aAssessment of Immunomodulatory and anti-parasitic effect of bee venom-loaded pluronic F-127 nanoparticles on schistosomiasis infection in CD1 mice /
_cAlaa Hussien Saleh Bashry ; Supervised Noha Ahmed Mahana , Abeer Mahmoud Badr , Ahmed Saeed Abdelmawgood
246 1 5 _aتقييم التأثير المناعى واالمضاد للطفليات لجزيئات البلوزونيك ف - 127النانونية المحملة بسم النحل على عدوى البلهارسيا فى فئران س د 1
260 _aCairo :
_bAlaa Hussien Saleh Bashry ,
_c2021
300 _a107 P . :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520 _aBackground: Honey-bee venom (BV) was reported to have an anti-schistosomiasis activity; however, it expands the hepatic granuloma size in high concentration. Aim: is to overcome the high-dose problems of BV by encapsulation inside pluronic F127 (F127) nanoparticles (NPs) and preserving the sustained release of BV. Methods: The treatment efficiency of free BV, F127 NPs or BV-loaded F127 NPs was investigated via in-vitro experiments and Shistosoma (S.) mansoni infected mice. After three weeks of treatment against S. mansoni either at the juvenile or adult stages. In addition, the parasitological, immunological and histopathological parameters were evaluated. Results: The obtained data showed a reduction in the total worm burden, oogram pattern and ova count for free BV, F127 NPs or BV-loaded F127 NPs compared to infected control. The immunomodulatory function was observed by stimulation of innate immune responses (IL-33) and T helper (Th)-1 immunity (TNF-Ü) when treated with loaded NPs while F127 NPs elicited the release of IL-33, TNF-Ü, IFN-Þ, IL-10 and IL-13 compared to infected control. All tested treatments induced a reduction in both the number and the diameter of granuloma, while BV-loaded NPs provoked newly-formed bile ductules. Both F127 NPs and BV-loaded NPs induced a significant decrease in liver fibrosis area percentage with respect to the infected control. Conclusion: Free BV has a limited effect on the larval stages of S. mansoni, while BV-loaded NPs and F127 NPs showed remarkable schistosomicidal effects with lower side effects
530 _aIssued also as CD
653 4 _aBee venom
653 4 _aPluronic F127 nanoparticles
653 4 _aSchistosoma mansoni
700 0 _aAbeer Mahmoud Badr ,
_eSupervising
700 0 _aAhmed Saeed Abdelmawgood ,
_eSupervising
700 0 _aNoha Ahmed Mahana ,
_eSupervising
856 _uhttp://172.23.153.220/th.pdf
905 _aAmira
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c81370
_d81370