| 000 | 02867cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223032757.0 | ||
| 008 | 210731s2021 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.07.10.M.Sc.2021.Ha.S | ||
| 100 | 0 | _aHager Mohsen Hassan Ali Khalil | |
| 245 | 1 | 0 |
_aStudies on gene regulation of some genes that related to bt. toxins resistance in the cotton worm, spodoptera littoralis / _cHager Mohsen Hassan Ali Khalil ; Supervised Nagwa Ibrahim Abdelfattah Elarabi , Dalia Sayed Ahmed Hasaneen , Saad Mohamed Moussa |
| 246 | 1 | 5 | _aSpodoptera littoralisفى دودة ورق القطن (Bt. Toxins) دراسات حول التنظيم الجينى لبعض الجينات التى لها علاقة بالمقاومة ضد المبيدات |
| 260 |
_aCairo : _bHager Mohsen Hassan Ali Khalil , _c2021 |
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| 300 |
_a93 P. : _bcharts , facimiles ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics | ||
| 520 | _aA better understanding of the processing of Bacillus thuringiensis (Bt) Cry1C toxin in the midgut of Spodoptera littoralis larvae is very important to characterize the main regulatory elements of Bt tolerance. The current study aimed to evaluate the mRNA level of trypsin (Try), aminopeptidase N (APN), alkaline phosphatase (ALP), cadherin (Cad), and cytochrome P450 (CYP) in both susceptible and Cry1C- tolerant strains of S. littoralis. Total RNA was extracted from susceptible and tolerant strains to construct cDNA. Quantitative real-time PCR (qPCR) showed a significant upregulation of CYP gene in tolerant strain. In contrast, the levels of expression of Try, ALP and Cad were significantly downregulated in tolerant strain. APN relative mRNA expression did not show significant differences between susceptible and tolerant strains. Histologically, the midgut of late third-instar larvae of tolerant population S. littoralis showed vacuolization of the epithelium and disruption of both the peritrophic membrane and the striated boarder compared to the susceptible strain. Our data indicate the important roles of CYP, Try, ALP and Cad in the resistance development and toxicity to Bt Cry1C.The obtained results are useful for further illustrating of Bt Cry1C processing and S. littoralis tolerance | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aBacillus thuringiensis | |
| 653 | 4 | _aBt Cry1C toxin | |
| 653 | 4 | _aSpodoptera littoralis | |
| 700 | 0 |
_aDalia Sayed Ahmed Hasaneen , _eSupervisor |
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| 700 | 0 |
_aNagwa Ibrahim Abdelfattah Elarabi , _eSupervisor |
|
| 700 | 0 |
_aSaad Mohamed Moussa , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
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| 905 |
_aShimaa _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c81525 _d81525 |
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