000			02977cam a2200349 a 4500
003			EG-GiCUC
005			20250223032834.0
008			211024s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.05.M.Sc.2021.Da.A
100	0		_aDalia Omar Abdelazem Elsafty
245	1	0	_aAntimicrobial studies of citrullus colocynthis collected from different ecological habitats in Egypt / _cDalia Omar Abdelazem Elsafty ; Supervised Mohamed Ibrahim Ahmed Ali , Salama A. Ouf , Maha G. Haggag
246	1	5	_aدراسة تأثير الحنظل كمضاد للميكروبات من بيئات مختلفة فى مصر
260			_aCairo : _bDalia Omar Abdelazem Elsafty , _c2021
300			_a124 P. : _bcharts , facsimiles , maps ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology
520			_aAqueous and methanolic seed extracts of Citrullus colocynthis from different geographical habitats, namely Sinai, ElWadi ElGedid, and Wadi Qena, were screened for antimicrobial activities.The tested microorganisms include 18 species, of which 6 bacteria, 6 Candida, and 6 dermatophyte fungi. The methanolic extract was more effective than aqueous extract for all microorganisms.The extracts were variably effective with more significant inhibition towards dermatophytes, particularly the seeds from Sinai.The effect of methanol seed extract from Sinai combined with 0.5 og/ml fluconazole act as a synergistic factor in decreasing spore viability, reduction of keratinase, elastase, lipase, phospholipase, and protease was more pronounced. The phytochemical analyses of the methanolic seed extracts from the three localities shows the presence of Hexadecanoic acid, methyl ester, 9,12- Octadecadienoic acid (Z,Z), and 9,12-Octadecadienoic acid (Z,Z)-, 2-hydroxy-1- (hydroxymethyl) ethyl ester as common compounds.The in-silico docking activity of 9,12-Octadecadienoic acid (Z,Z), Methyl 9-cis,11-trans-octadecadienoate, and 9,12-Octadecadienoic acid, methyl ester, (E,E) toward keratinase and lipase enzymes of Microsporum canis and Trichophyton mentagrophytes showed a high affinity of binding between 9,12-Octadecadienoic acid (Z,Z) and lipase enzyme of Microsporum canis and Trichophyton mentagrophytes as it forms 3 H-bonds with Gln 106, Ser 107, and Thr 102 of Microsporum canis lipase, in addition, it forms 4 H-bonds with Gln 129, Gly 124, Ile 128, and Ser 130 of Trichophyton mentagrophytes lipase
530			_aIssued also as CD
653		4	_aAntimicrobial activity
653		4	_aBacteria
653		4	_aCitrullus colocynthis
700	0		_aMaha G. Haggag , _eSupervisor
700	0		_aMohamed Ibrahim Ahmed Ali , _eSupervisor
700	0		_aSalama A. Ouf , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c82754 _d82754