| 000 | 01860cam a2200337 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223032839.0 | ||
| 008 | 211031s2021 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.12.05.M.Sc.2021.Ta.C | ||
| 100 | 0 | _aTaher Talal Mahrous Ahmed | |
| 245 | 1 | 0 |
_aCloning, expression and characterization of bacterial L - glutaminase / _cTaher Talal Mahrous Ahmed ; Supervised Zienat Kamel Mohamed Ibrahim , Mohamed G. Farahat |
| 246 | 1 | 5 | _aاستنساخ و تعبير و توصيف ل- جلوتامينيز البكتيرى |
| 260 |
_aCairo : _bTaher Talal Mahrous Ahmed , _c2021 |
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| 300 |
_a84 P. : _bcharts , fascimiles ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology | ||
| 520 | _aL-Glutaminase has received an increased importance in recent years for its anti-carcinogenic potential. In this study, Klebsiella michiganensis was isolated from the Lake Mariout and the glsA gene encoding L-glutaminase from was cloned into pQE-80L expression vector and expressed in E. coli BL21.The recombinant histidine-tagged L-glutaminasewas purified to homogeneity by Ni{u2013}NTAaffinity chromatography and displayed a single 33.0 kDa band on SDS-PAGE.The purified functional enzyme exhibited a specific activity at 89 U/mg and inhibited the growth of human colon cancer cells (HCT116) with IC50 value of 2.4±0.7 U/ml | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _a L-Glutaminase | |
| 653 | 4 | _aCloning | |
| 653 | 4 | _aScreening | |
| 700 | 0 |
_aMohamed G. Farahat , _eSupervisor |
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| 700 | 0 |
_aZienat Kamel Mohamed Ibrahim , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
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| 905 |
_aShimaa _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c82891 _d82891 |
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