000 03316cam a2200349 a 4500
003 EG-GiCUC
005 20250223032844.0
008 211108s2021 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.07.04.Ph.D.2021.Ra.B
100 0 _aRasha Mohamed Mohamed Ali Mohasib
245 1 0 _aBiochemical studies on chenopodium murale and sonchus oleraceus plants /
_cRasha Mohamed Mohamed Ali Mohasib : Supervised Abdel Moneim Mhomed Naguib , Abdelkader Moursy Elsayed Abd Elsamad , Zeinab Hanem Abdelrahman Salama
246 1 5 _a(Sonchus oleraceus)والجعضيض (Chenopodium murale)دراسات كيميائية حيوية على نباتات الزربيح
260 _aCairo :
_bRasha Mohamed Mohamed Ali Mohasib ,
_c2021
300 _a171 P. :
_bcharts ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Biochemistry
520 _aChenopodium murale and Sonchus oleraceus aerial parts were subjected to a successive extraction by using solvent of different polarities; each fraction was evaluated for its antioxidant, antibacterial, anticancer and the anti-arthritic activity. Each active extract was farther biogided fractionated to determine and identify the active compound/s present in each sub-fraction. All the tested species were extracted using four solvents: hexane, ethyl acetate, methanol and water. Phenols, flavonoids, and tannins were quantified for all extracts.The antioxidant activity was measured by using four methods: DPPH., ABTS., Fe⁺²and chelating reducing power for the four fractions of the plants under study.The best values of IC₅₀ were obtained for their ability to neutralize each of DPPH., ABTS.,Fe⁺²and chelating reducing power from ethyl acetate extract and methanol. Ethyl Acetate and methanol extracts showed higher antibacterial activity (gram-positive and gram-negative) than the aqueous extract of both C. murale and S. oleraceus.The results also showed anti-tumor activity against breast, colon and liver cancer cells. The anti-growth activity of tumors depends on the concentration extracted using the MTT method.The anti-inflammatory activity results showed an increase in inhibition with increasing the concentration of plant extracts. For C. murale and S. oleraceus, ethyl acetate fraction was found to be the most important as it produced the highest percentage of inhibition, compared to Diclofenac at 500 mg / mL. Regarding the C. murale plant, the study revealed the highest inhibition rate of methanolic fraction, followed by hexane fraction. The identification of chemical constituents of each fraction was carried out by GC/MS analysis for non-polar, methylated fractions and HPLC techniques were used for analysis of polar fractions
530 _aIssued also as CD
653 4 _aAnti-arthritic activities
653 4 _aAnti-proliferative
653 4 _aAntioxidant
700 0 _aAbdel Moneim Mhomed Naguib ,
_eSupervisor
700 0 _aAbdelkader Moursy Elsayed Abdelsamad ,
_eSupervisor
700 0 _aZeinab Hanem Abdelrahman Salama ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c83057
_d83057