000			03067cam a2200349 a 4500
003			EG-GiCUC
005			20250223032847.0
008			211114s2021 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.12.02.Ph.D.2021.Ah.C
100	0		_aAhlam Mohamed Abdelaziz Mohamed
245	1	0	_aCardiprotective effect of pentoxify lline in ischemic heart mediated via blockage of immune checkpoints / _cAhlam Mohamed Abdelaziz Mohamed ; Supervised Mohamed Ali Eldesouky , Ayman Elsayed Shafei Abouelaa , Mostafa Mahmoud Mohamed Elnakib
246	1	5	_aالتأثير الوقائى للبنتوكسيفيلين لمرض نقص تروية القلب عن طريق إحداث صمت فى نقاط التفتيش المناعية
260			_aCairo : _bAhlam Mohamed Abdelaziz Mohamed , _c2021
300			_a162 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry
520			_aThe regulation by immune checkpoints is able to prevent excessive tissue damage caused by ischemia/reperfusion (I/R); therefore, the study aims to investigate the behavior of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) mRNA, miR-1206 and small nucleolar RNA host gene 14 (SNHG14) during I/R and intake of pentoxifylline (PTX) as a protective drug.The relative expression level of PAG1/miR- 1206/SNHG14 was determined by qRT-PCR. Cardiac tissue levels of cytotoxic T-lymphocyte associated antigen 4 (CTLA4) and PAG1 protein expression were determined by ELISA technique. The regulatory T cells were achieved by the flow cytometry. The results indicated that the relative expression of SNHG14 was significantly up regulated in I/R, but suppressed in PTX treated groups with enhancement of the relative expression level of miR-1206. The gene and protein expression of PAG1 were down regulated with effective doses of PTX.The results showed that PTX dose between 30 and 40 mg/kg suppressed the CTLA4 development significantly.The mean of the regulatory T cell in PTX protective groups was significantly reduced at (p < 0.001) in a comparison with I/R group. Spearman{u2019}s correlation analysis revealed a significant negative correlation between SNHG14 and miR-1206, while a significant positive correlation between SNHG14 and PAG1 in I/R heart tissue. Based on the obtained results it is concluded that miR-1206 and SNHG14 may be used as biomarkers with perfect sensitivity and specificity in I/R. Further of PTX also reduced cardiac tissue damage
530			_aIssued also as CD
653		4	_aIschemia reperfusion
653		4	_aMiR-1206
653		4	_aPentoxifylline
700	0		_aAyman Elsayed Shafei Abouelaa , _eSupervisor
700	0		_aMohamed Ali Eldesouky , _eSupervisor
700	0		_aMostafa Mahmoud Mohamed Elnakib , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c83130 _d83130