000			03145cam a2200349 a 4500
003			EG-GiCUC
005			20250223032900.0
008			211213s2021 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.19.02.Ph.D.2021.Es.P
100	0		_aEsraa Aly Zeen Elabdeen Khalil
245	1	0	_aPotential therapeutic efficacy of certain natural products against colorectal cancer cells / _cEsraa Aly Zeen Elabdeen Khalil ; Supervised Wafaa Ahmed Abdullah , Saad Elguendy , Amany Abdelhameed
246	1	5	_aالتأثير العلاجى المحتمل لبعض النباتات الطبيعيه لعلاج سرطان القولون والمستقيم
260			_aCairo : _bEsraa Aly Zeen Elabdeen Khalil , _c2021
300			_a122 P. : _bcharts ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology
520			_aColorectal cancer is the third most common cancer, but it is the second most deadly cancer worldwide. In Egypt, Colorectal cancer is Egypt's seventh most prevalent cancer, accounting for 3.47 percent of male cancers and 3% of female cancers. Jasonia Montana is one of the most prevalent therapeutic herbs. The bio medicinal action of Jasonia montana is due to a variety of active compounds that combine to form the complex impact of the plant. Quercetin is one of the important flavonoids that exert anti{u2010}proliferative effects on cancer cells mediated by various mechanisms of action including alterations in gene expression of oncogenes, tumor suppressor genes. MiRNAs are short, non-protein-coding RNAs {u223C}22 nucleotides in length that are known to alter gene expression at a post-transciptional level. Here, we investigated the potential efficacy of jasonia Montana to trigger colon cancer cells toward differentiation. In this study we fulfilled miRNAs& their targeted genes related to differentiation pathways such as NOTCH, PI3K/AKT & pluroipotancy gene SOX2 in both CACOO2&HCT116 colon cell lines and then evaluate stem cell marker CD44& differentiation marker CK20 to ensure the efficacy of jasonia Montana toward differentiation. Using syber green RT-qPCR , both miR-21& miR-10B were shown to be significantly downregulated While their targeted gene PTEN (which included in PI3K/AKT pathway) shown to be significantly upregulated . On the other hand, miR-34a was significantly up regulated &its targeted gene NOTCH1 was down regulated,miR-145 was upregulated while its targeted gene SOX2 was down regulated. Finally, CD44 as stem cell marker was downregulated while CK20 as differentiation marker was upregulated. In conclusion, jasonia Montana may be used as
530			_aIssued also as CD
653		4	_aColorectal cancer
653		4	_aJasonia montana
653		4	_amiRNAs
700	0		_aAmany Abdelhameed , _eSupervisor
700	0		_aSaad Elguendy , _eSupervisor
700	0		_aWafaa Ahmed Abdullah , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c83557 _d83557