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005			20260517111229.0
008			220305s2022 ua dh frm 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a580
092			_a580 _221
097			_aPh.D
099			_aCai01.12.05.Ph.D.2022.Ha.P
100	0		_aHanan Mohammed Kamal Abdelfatah Osman, _epreparation.
245	1	0	_aProduction and characterization of microbial myrosinase / _cby Hanan Mohammed Kamal Abdelfatah Osman ; Supervised Prof.Dr. Mary Sobhy Khalil, Prof.Dr. Sameh Heikal Youseif.
246	1	5	_aانتاج وتوصيف الميروسينيز الميكروبى
264		0	_c2022
300			_a162 P. : _bcharts , facsimiles ; _c25cm+ _eCD
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany Microbiology
504			_aBibliography: Pages 191-228.
520			_aMyrosinase hydrolyzes glucosinolates giving various products depending on the physiological conditions.The hydrolysis products especially isothiocyanates have antibacterial, antifungal, anticancer activity, act as soil biofumigant, natural pesticides and food preservatives. Myrosinase is mainly found in cruciferous plants but few literatures reported its production by both fungi and bacteria. In this study, 94 microbial isolates from E.sativa and its soil rhizosphere were screened for myrosinase, 25% of the screened bacterial isolates (9 isolates) and 62.07% of the screened fungal isolates (36 isolates) had myrosinase activity. About 22.22% of the endophytic bacterial isolates from E.sativa roots and 63.63% of the endophytic bacterial isolates from E.sativa leaves were able to produce myrosinase but none of soil rhizosphere and epiphytic bacterial isolates were able. On the other hand, 72.41% of the E.sativa fungal isolates of soil rhizosphere, 50% of the endophytic fungal isolates from E.sativa roots, 37.5% of the endophytic fungal isolates from E.sativa leaves and 63.64% of the epiphytic isolates had myrosinase activity.This indicated that both endophytic bacterial and fungal population of E.sativa leaves and roots are good reservoir for myrosinase activity and the fungal community of the soil rhizosphere and the plant surfaces of E.sativa seemed to have myrosinase activity rather than the bacteria living there. The nine myrosinase producing bacterial isolates were phenotypically characterized and identified based on 16S rRNA sequencing.They all belonged to the genus Bacillus and they were given the following accession numbers LC589981, LC589982, LC589983, LC589984, LC589985, LC589986, LC589987, LC589988 and LC589989 on NCBI. They were all most closely related to Bacillus siamensis and Bacillus velezensis with identity percent > 99%. The fungal myrosinase producers were identified morphologically and they belonged to six different genera: Aspergillus, Penicellium, Fusarium, Eumericella, Alternaria and Sclerotium
530			_aIssued also as CD
546			_aText in English and abstract in Arabic & English.
650		0	_aMicrobiology
653		1	_aEruca sativa _aFungi _aMyrosinase
700	0		_aMary Sobhy Khalil _ethesis advisor.
700	0		_aSameh Heikal Youseif _ethesis advisor.
900			_b01-01-2022 _cMary Sobhy Khalil _c Sameh Heikal Youseif _UCairo University _FFaculty of Science _DDepartment of Botany and Microbiology
905			_aShimaa _eRevisor
942			_2ddc _cTH
999			_c84411