Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Physiology

The role of insulin and its status are key components in metabolic syndrome. Impressive body of epidemiological data collected over the past decade indicates that a disturbing link may connect the insulin status, to a pre-neoplastic state which may fortell an additional danger: cancer. This evidence also includes studies that examined in particular, the risk of colon cancer or adenoma in relation to some significant determinants of metabolic syndrome related to the insulin IGF 1 axis. Thus, the present study aimed to further explore the possible role of insulin, IGF-1 axis in modulating the pathogenic events related to the low grade chronic pre-neoplastic inflammation induced experimentally in the colon of a diabetic female rat model. To achieve this aim, two experimental diabetic models were induced. The first experimental model was applied to a group of female rats to induce hyperinsulinemia (T2DM) by keeping them on a diet of high glycemic value ad lipidum. The second was induced on a second group of animals to induce a state of hypoinsulinemia (T1DM) by giving them a single dose of streptozotocin. Study groups included: a control group representing the vehicle treated rats (Group I) and two main experimental groups: T2DM (Group II) and T1DM (Group III). All animals were left for a study period of 8 weeks. Four weeks before the termination of the study period, the animals of the three groups were further divided into 2 subgroups: the A subgroups (IA, IIA, IIIA) were slowly infused with a single dose of saline into the distal colon, the B subgroups (IB, IIB, IIIB) were subjected to a single intracolonic dose infusion of 4% acetic acid solution

Issued also as CD