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Study the effect of nano and micro encapsulation of DNA vaccine model on its stability and immunogenicity /

Heba Ibrahim Mohamed Abdallah

Study the effect of nano and micro encapsulation of DNA vaccine model on its stability and immunogenicity / دراسة تأثير الصياغة على الثبات والقدرة المناعية لنموذج لقاح حامض الديكسيريبوز فى صورة حويصلات دقيقة او نانو مترية Heba Ibrahim Mohamed Abdallah ; Supervised Mahmoud Mohamed Ghorab , Aly Fahmy Mohamed - Cairo : Heba Ibrahim Mohamed Abdallah , 2017 - 172 P. : charts , facsimiles ; 25cm

Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmaceutics

Herpes simplex virus type 2 (HSV-2) is a one of the most prevalent worldwide sexually transmitted infection. HSV-2 causes recurrent genital ulcers, neonatal herpes, and also, increases the risk for human immunodeficiency virus (HIV) acquisition. Many HSV-2 vaccines have been tested in humans, but were not consistently effective. In the present work we tried to produce a model vaccine for HSV-2 formulated in different formulae as an attempt for development of an effective prophylactic and therapeutic HSV-2 vaccine. Beta propiolactone (BPL) as a virus inactivant was compared with the conventional formalin (F). Gold and silver nanoparticles were prepared with different concentrations as a vaccine adjuvants. Also, calcium phosphate nanoparticles and bovine serum albumin nanoparticles were used as vaccine adjuvants . They were compared with currently used aluminum phosphate gel (alum) microparticles



DNA DNA vaccine model Nano and micro encapsulation