Mitochondrial DNA microchimerism in kidney transplant recipients /
Mahmoud Sayed Zidan Abdelall
Mitochondrial DNA microchimerism in kidney transplant recipients / الكشف عن الخيمرية الدقيقة باستخدام الحامض النووى فى مستقبلى زراعة الكلى Mahmoud Sayed Zidan Abdelall ; Supervised Mohamed Gamal Eldin Saadi , May A. Hassaballa , Mervat Elansary - Cairo : Mahmoud Sayed Zidan Abdelall , 2017 - 123 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Internal Medicine
Microchimerism is the presence of a small number of cells that originate from another individual and are therefore genetically distinct from the cells of the host individual, microchimerism has been proposed to play a benecial immunomodulatory role toward the development of donor-specic hypo responsiveness and allograft acceptance. Objectives: In this study, a quantitative amplification refractory mutation system quantitative polymerase chain reaction (ARMS-qPCR) approach using mitochondrial (mtDNA) polymorphisms of the HVRs IIII of the D-loop region of mtDNA was used to detect donor-specic microchimerism tracing circulating mtDNA molecules in peripheral mononuclear blood cells ( PBMCs ) of renal transplant recipients, and to correlate between the presence or absence of donor-specic microchimerism and graft survival and function as measured using serum creatinine and creatinine clearance. Patients and methods: ARMS-qPCR approach using mitochondrial DNA polymorphisms was used to detects and quantitates donor-specic microchimerism with correlatation with graft function. Results: Donor-specific mtDNA using ARMS-qPCR technique was detectable in 17 cases (85%) ranging from 0.001% to 1.67% with mean value of 0.3638±0.3981%. Microchimerism was associated with early better graft function in spite of no impact on later graft function. Conclusion: We successfully used a sensitive real-time ARMS-PCR method based on mitochondrial DNA to determine and quantitate the donor microchimerism in kidney transplant recipients. Microchimerism was detected after kidney transplantation and was associated with early better graft function inspite of no impact on later graft function
Kidney transplant recipients Microchimerism Mitochondrial DNA
Mitochondrial DNA microchimerism in kidney transplant recipients / الكشف عن الخيمرية الدقيقة باستخدام الحامض النووى فى مستقبلى زراعة الكلى Mahmoud Sayed Zidan Abdelall ; Supervised Mohamed Gamal Eldin Saadi , May A. Hassaballa , Mervat Elansary - Cairo : Mahmoud Sayed Zidan Abdelall , 2017 - 123 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Internal Medicine
Microchimerism is the presence of a small number of cells that originate from another individual and are therefore genetically distinct from the cells of the host individual, microchimerism has been proposed to play a benecial immunomodulatory role toward the development of donor-specic hypo responsiveness and allograft acceptance. Objectives: In this study, a quantitative amplification refractory mutation system quantitative polymerase chain reaction (ARMS-qPCR) approach using mitochondrial (mtDNA) polymorphisms of the HVRs IIII of the D-loop region of mtDNA was used to detect donor-specic microchimerism tracing circulating mtDNA molecules in peripheral mononuclear blood cells ( PBMCs ) of renal transplant recipients, and to correlate between the presence or absence of donor-specic microchimerism and graft survival and function as measured using serum creatinine and creatinine clearance. Patients and methods: ARMS-qPCR approach using mitochondrial DNA polymorphisms was used to detects and quantitates donor-specic microchimerism with correlatation with graft function. Results: Donor-specific mtDNA using ARMS-qPCR technique was detectable in 17 cases (85%) ranging from 0.001% to 1.67% with mean value of 0.3638±0.3981%. Microchimerism was associated with early better graft function in spite of no impact on later graft function. Conclusion: We successfully used a sensitive real-time ARMS-PCR method based on mitochondrial DNA to determine and quantitate the donor microchimerism in kidney transplant recipients. Microchimerism was detected after kidney transplantation and was associated with early better graft function inspite of no impact on later graft function
Kidney transplant recipients Microchimerism Mitochondrial DNA