MARC details
| 000 -LEADER |
|---|
| fixed length control field |
01917cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
150122s2014 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.12.05.Ph.D.2014.Mo.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohamed Gamal Salah Farahat |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Expression and purification of recombinant histidine-tagged L-asparaginase II from escherichia coli / |
| Statement of responsibility, etc. |
Mohamed Gamal Salah Farahat ; Supervised Zienat Kamel Mohamed Ibrahim , AlaaEddeen Seufi Mohamed , Sherif Mohamed Elnagdy |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
معاد الاتحاد المعلم بالهيستيدين من الايشيريشيا كولاى IIتعبير وتنقية ل-اسبراجيناز |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Mohamed Gamal Salah Farahat , |
| Date of publication, distribution, etc. |
2014 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
178 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany Microbiology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
L-asparaginase (L-ASNase) has been widely used as a therapeutic agent in the treatment of various lymphoblastic leukemia diseases. In this study, Escherichia coli MG27 was isolated from the River Nile and subjected to optimization studies to maximize the production of L-ASNase. ansB gene, encoding L-ASNase II from E. coli MG27, was cloned into pQE-30 expression vector and expressed in E. coli M15. The recombinant histidine-tagged L-ASNase II was purified to homogeneity by Ni{u2013}NTA affinity chromatography and displayed a single 36.0 kDa band on SDS-PAGE |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
L-Asparaginase |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Optimization |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Screening |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Alaa Eddeen Seufi Mohamed , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Sherif Mohamed Elnagdy , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zienat Kamel Mohamed Ibrahim , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Aml |
| Reviser |
Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
