Expression and purification of recombinant histidine-tagged L-asparaginase II from escherichia coli / Mohamed Gamal Salah Farahat ; Supervised Zienat Kamel Mohamed Ibrahim , AlaaEddeen Seufi Mohamed , Sherif Mohamed Elnagdy

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Mohamed Gamal Salah Farahat

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TextLanguage: English Publication details: Cairo : Mohamed Gamal Salah Farahat , 2014Description: 178 P. : charts , facsimiles ; 25cmOther title:

معاد الاتحاد المعلم بالهيستيدين من الايشيريشيا كولاى IIتعبير وتنقية ل-اسبراجيناز [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany Microbiology Summary: L-asparaginase (L-ASNase) has been widely used as a therapeutic agent in the treatment of various lymphoblastic leukemia diseases. In this study, Escherichia coli MG27 was isolated from the River Nile and subjected to optimization studies to maximize the production of L-ASNase. ansB gene, encoding L-ASNase II from E. coli MG27, was cloned into pQE-30 expression vector and expressed in E. coli M15. The recombinant histidine-tagged L-ASNase II was purified to homogeneity by Ni{u2013}NTA affinity chromatography and displayed a single 36.0 kDa band on SDS-PAGE

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Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.05.Ph.D.2014.Mo.E (Browse shelf(Opens below))		Not for loan		01010110064452000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.05.Ph.D.2014.Mo.E (Browse shelf(Opens below))	64452.CD	Not for loan		01020110064452000

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany Microbiology

L-asparaginase (L-ASNase) has been widely used as a therapeutic agent in the treatment of various lymphoblastic leukemia diseases. In this study, Escherichia coli MG27 was isolated from the River Nile and subjected to optimization studies to maximize the production of L-ASNase. ansB gene, encoding L-ASNase II from E. coli MG27, was cloned into pQE-30 expression vector and expressed in E. coli M15. The recombinant histidine-tagged L-ASNase II was purified to homogeneity by Ni{u2013}NTA affinity chromatography and displayed a single 36.0 kDa band on SDS-PAGE

Issued also as CD

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