A pharmaceutical study on certain solid lipid nanoparticles by Yomna Ahmed Moussa Abdelrahman ; supervision of Prof. Mohamed Ahmed EL Nabarawi, Prof. Dalia Abd El Rahman Attia , Prof. Mohamed Mohey Eldein Elmazar , Prof. Mahmoud Hassan Teaima

Thesis (Ph.D)-Cairo University, 2023.

Bibliography: pages 271-314.

Green coffee bean extract (GCBE), derived from unroasted coffee beans, has garnered considerable attention in recent years due to its rich composition of bioactive compounds. The main active component of GCBE is chlorogenic acid (CGA), a potent polyphenol known for its various biological activities. GCBE has demonstrated promising effects in the management of several health conditions, including antiobesity, anticelluite, antioxidant, antihypertension, and anti- inflammatory. Furthermore, it has been examined for its repurposed use as anticancer in HCT116 (colon cell line), HepG2 (liver cell line) and A649 (lung cancer cell line).
Despite the various health benefits of CGA. Previous studies proved that CGA has an overall limited intestinal absorption that involves an active efflux mechanism that results in low bioavailability of chlorogenic acid. So, it was necessitated to incorporate GCBE in a valid dosage form as until now there hasn’t been any approved FDA GCBE product in the market.
Solid lipid nanoparticles (SLNs) have emerged as a promising nanocarrier system for the delivery of various therapeutics. As they offer several benefits such as controlled release, enhanced bioavailability, biocompatibility, and site-specific accumulation, which make them an attractive option for various applications. In addition, the ability to modulate the physicochemical properties of drug loaded SLNs, such as particle size, surface charge, and entrapment efficiency, allows the development of tailored drug delivery systems with controlled release capabilities.
So, the aim of this work was to enhance the therapeutic effects of GCBE by loading GCBE into SLNs and assess its activity compared to non-formulated GCBE



which can be achieved by screening and optimizing GCBE-SLNs using Plackett- Burman design and Box-Behnken design to reach optimized formula of GCBE- SLNs that was assessed for its absorption and topical applications including its repurposed application as an anticancer drug in vitro and in vivo, as well as its traditional application as an anticellulite drug using clinical study.

مستخلص حبوب القهوة الخضراء ، المستمد من حبوب القهوةغير المحمصة، لفت انتباها كبيرا في السنوات الأخيرة بسبب تركيبته الغنية من المركبات الحيوية. المكون النشط الرئيسي في مستخلص حبوب القهوة الخضراء هو حمض الكلوروجينيك، وهو مركب بوليفينولي قوي معروف بأنه له أنشطة حيوية متنوعة. أظهر مستخلص حبوب القهوة الخضراء تأثيرات مشجعة في علاج العديد من الحالات الصحية كمضاد للسمنة، ومكافح للسلوليت، ومضاد للأكسدة، ومضاد لارتفاع ضغط الدم، ومضاد للالتهاب.
هدف الرساله كان تعزيز توصيل وقابلية امتصاص مستخلص حبوب القهوة الخضراء ، من خلال تحميل مستخلص حبوب القهوة الخضراء في جزيئات الدهون الصلبة النانومترية ثم دمجه في كريم مما يعد استراتيجية واعدة لتطوير علاج ناجح. فرضية هذا العمل أن حمل مستخلص حبوب القهوة الخضراء داخل جزيئات الدهون الصلبة النانومترية يمكن أن يحميه من التحلل، ويسهل إطلاقه المنظم، ويحسن قابلية امتصاصه عند التناول عن طريق الفم ويمكنه من التراكم النوعي في الأنسجة أو الخلايا المستهدفة عند التطبيق الموضعي، وبالتالي يتم تحسين إمكاناته العلاجية.

Issues also as CD.

Text in English and abstract in Arabic & English.