Molecular and biochemical studies on osteoarthritis in equine/ Huda Omar Abubaker ; Supervised Hatim Mohamed Ahmed Elhindi , Said Zaki Mousa , Adel M. Abouelfetouh Elbehairy

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Biochemistry and Chemistry of Nutrition

A central feature of the osteoarthritis (OA) involves erosive destruction of the articular cartilage extracellular matrix (ECM) on the surfaces of joints. The resultant loss of joint function makes studies on mechanisms underlying ECM degradation critical for treatment of the disease and prevention of disability. In the present study serum and synovial fluid samples were collected from normal (n=20), Early OA (n=10) and Late OA (n=20) adult male horses attended from surgery clinic, faculty of veterinary medicine Cairo University. The concentrations of total (TGAGs), individual (CS) and non sulphated (HA) glycosaminoglycans, Procollagen IIC-propeptide (PIICP) and Tartarate resistant acid phosphatase(TRAP) activities were estimated in serum and synovial fluid .The activities of different types of matrix metalloproteinases (MMPs) and tissue inhibitor metalloproteinases (TIMPs) in synovial fluid were estimated by Substrate and Reverse zymography respectively and the expression of MMP-13 was determined by Western blot. The allele and genotype frequencies of the Growth differentiation factor 5(GDF5) and Asporin (ASPN) genes were estimated by PCR- SSCP

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