Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Dermatology and Venerology

Patients and methods: This observastional case control study was conducted on 100 patients with vitiligo and 80 age and sex matched controls. From each A 5ml blood sample was taken for detection of CBS (844ins68) gene polymorphism by PCR and detection of Hcy level by Axis Homocysteine Enzyme Immunoassay kit. Results: A significant higher frequency of the CBS (844ins68) gene polymorphism (homozygous or heterozygous) was found in the patient group in relation to the control group (25% vs 12.5% with P = 0.035*). A statistically significant difference was found between cases with normal genotype and those with mutant genotype as regards family history (p = 0.000*). Also a statistically significant difference was found between cases and controls as regards Hcy level (p = 0.000*). However no statistically significant difference was found between cases with normal genotype and those with mutant genotype as regards the age (P = 0.506.), extent (P = 0.655), age of onset (P = 0.097), duration of illness (P = 0.179), VIDA (P = 0.412),VASI (p = 0.905) and Hcy level (p = 0.403). A significant correlation was found between Hcy level and age of patients, disease duration, extent of disease and VASI score p (0.033), (0.021) , (0.000), (.000) respectively. Non significant correlation was found between Hcy level and CBS (844ins68) genotypes and VIDA (p = 0.232), (p = 0.103) respectively. Conclusion: We concluded that CBS gene polymorphism may paly an additional role in the susceptibility of individuals for vitiligo

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