Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Out of 150 samples (50 from imported chicks,ducklings), (50 from imported frozen chicken carcasses) and (50 from native poultry) the incidence of campylobacter was 11.33%. Campylobacter was isolated from fecal matter of imported chicks,drip of frozen chicken carcasses and cloacal swabs of native poultry and it was found that the highest percent of isolation was from native poultry16% followed by imported birds 14%,while the lowest isolation rate was from frozen chicken carcasses 4%. By cultivationof 6 isolates of Campylobacter by three different media (mCCDA, karmali, preston), it was found that mCCDA medium was the most effective medium for enumeration of campylobacter followed by karmali then preston. An inter laboratory experiment design was held to compare several techniques for detection of lowest number of campylobacter cells in chicken meat sample,it was found that enrichment in bolton broth followed by plating on mCCDA and PCR were able to detect as low as 10 cfu/gm of sample. Detection of 5 of genes (flaA) and (cdtA) and (virB11) and (iam) and (wlan) associated with virulence factors were done by PCR, and the study showed the the most prevalent gene was flaA gene70.6% followed by cdtA gene 47% then wlan 17.6% and iam 11.8%, while no virB gene could be found in this study. Also,in this study comparison of (i) cryobank microbial preservation system using(i) hypertonic {u2018}cryopreservative solution{u2019} and glass beads, (ii) FBP medium, (iii) 20% glycerol / 80% nutrient broth culture, and (iv) 50% glycerol / 50% nutrient broth (v) bovine and swine serums,and Bovine serum preservation technique proved the most successful preservation technique with best viability of the recovered cells for 160 days

Issued also as CD