Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

Cisplatin (CDDP) is a widely potent anticancer drug used in chemotherapy, unfortunately it elicits major undesirable side effects in various tissues, including liver and kidney. The aim of the present study was to investigate whether Origanum majorana ethanolic extract (OMEE) can reduce cisplatin-induced hepatorenal toxicity. The effectiveness of OMEE was compared with silymarin as a standard hepatoprotective drug. Male albino rats were divided into four groups (6 rats/group). Rats of the control group were administered distilled water for 17 consecutive days. The rest of rats were received a single intraperitoneal injection of CDDP (3 mg/kg body weight), then after 3 days, rats were administered orally for 14 days distilled water (CDDP group) or OMEE (500 mg/kg/day) (OMEE group) or silymarin (150 mg/kg/day). CDDP-induced hepatorenal toxicity was assessed biochemically by an increase in levels of serum ASAT, ALAT, ÞGT, LDH, ALP, total bilirubin, creatinine, urea, uric acid, BUN, total lipid, triglycerides and LDL-cholesterol. In addition, a decrease in serum total protein, albumin and HDL-cholesterol concentrations was recorded in comparison with the control group. Meanwhile, CDDP-induced an oxidative stress, which evidenced by a significant increase in hepatic and renal MDA, NO levels as well as a significant reduction in hepatic and renal GSH, SOD, GST and CAT contents as compared to the corresponding one of control group. Liver and kidney histopathological changes were observed in the cisplatin group as compared to the control group. Interestingly, oral administration of OMEE or silymarin significantly ameliorates the abnormal effects of CDDP on nearly all the studied parameters

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