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Molecular biological studies on Escherichia coli isolated from diarrheic and apparently healthy calves / Noha Mohamed Bakry Nady ; Supervised Walid Sayed Ahmed Abdelkader Awad , Amr Abdelaziz Abdelkader Elsayed

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Noha Mohamed Bakry Nady , 2016Description: 116 P. : charts , facsimiles ; 25cmOther title:
  • دراسات بيوجزيئية عن الايشيريشيا كولاى العزولة من عجول مصابة بالاسهال و عجول سليمة ظاهريا [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases Summary: Neonatal calf diarrhea is one of the most important problems in young calves causing great economic losses. In the current study, a total number of 150 fecal samples from diarrheic and apparently healthy in-contact cattle and buffalo calves under 3 months old were examined bacteriologically for isolation of E. coli. All isolates were confirmed by PCR using 16SrRNA gene. Multiplex PCR was applied for detection of virulence genes and for studying the association of these genes with different E. coli pathotypes. One hundred and six of 150 isolates were positive for at least one virulence gene. The rate of f , st, lt, eae, stx , stx , hylA and astA genes were 17.3%, 27.3%, 6.7%, 10%, 37.3%, 5 1 2 17.7%, 9.3% and 20.7%, respectively. Distribution of E. coli pathotypes revealed presence of ETEC, EPEC, STEC, EAEC, ET.EP. and ET.ST. at a rate of 12.7%, 2.7%, 30.7%, 7.3%, 2.7% and 14.7%, respectively. The rate of pathogenic E. coli infection in calves was higher in winter season and in the first weeks of life. Molecular serogrouping of E. coli isolates was carried using 10 O serogroups and revealed presence of O91 serogroup in the highest rate (62.7%), with higher rate of O26, O91, O121 and O45 in diarrheic calves. Comparing between molecular serogrouping and agglutination test revealed the ability of multiplex PCR to detect all isolates which were positive by agglutination and also 8 negative isolates. Five intestinal samples from four dead calves grossly showing congestion and distention of intestinal loops were bacteriologicaly positive for E. coli with higher rate of eae gene. Histopathological examination revealed heamorrhagic enteritis, shortening and fusion of intestinal villi, desquamation of lining epithelium of intestinal mucosa with congestion of blood capillaries and intense leucocytic infiltration
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.09.M.Sc.2016.No.M (Browse shelf(Opens below)) Not for loan 01010110070383000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.09.M.Sc.2016.No.M (Browse shelf(Opens below)) 70383.CD Not for loan 01020110070383000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases

Neonatal calf diarrhea is one of the most important problems in young calves causing great economic losses. In the current study, a total number of 150 fecal samples from diarrheic and apparently healthy in-contact cattle and buffalo calves under 3 months old were examined bacteriologically for isolation of E. coli. All isolates were confirmed by PCR using 16SrRNA gene. Multiplex PCR was applied for detection of virulence genes and for studying the association of these genes with different E. coli pathotypes. One hundred and six of 150 isolates were positive for at least one virulence gene. The rate of f , st, lt, eae, stx , stx , hylA and astA genes were 17.3%, 27.3%, 6.7%, 10%, 37.3%, 5 1 2 17.7%, 9.3% and 20.7%, respectively. Distribution of E. coli pathotypes revealed presence of ETEC, EPEC, STEC, EAEC, ET.EP. and ET.ST. at a rate of 12.7%, 2.7%, 30.7%, 7.3%, 2.7% and 14.7%, respectively. The rate of pathogenic E. coli infection in calves was higher in winter season and in the first weeks of life. Molecular serogrouping of E. coli isolates was carried using 10 O serogroups and revealed presence of O91 serogroup in the highest rate (62.7%), with higher rate of O26, O91, O121 and O45 in diarrheic calves. Comparing between molecular serogrouping and agglutination test revealed the ability of multiplex PCR to detect all isolates which were positive by agglutination and also 8 negative isolates. Five intestinal samples from four dead calves grossly showing congestion and distention of intestinal loops were bacteriologicaly positive for E. coli with higher rate of eae gene. Histopathological examination revealed heamorrhagic enteritis, shortening and fusion of intestinal villi, desquamation of lining epithelium of intestinal mucosa with congestion of blood capillaries and intense leucocytic infiltration

Issued also as CD

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