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Mutagenesis and protoplast fusion of arthrobacter sp. for improved glucose isomerase production / Heba Sayed Mohamad Mostafa ; Supervised Sobhy Mohamed Mohsen , Wael Ahmed Bazaraa , Nagwa Mohamed Elshimi

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Heba Sayed Mohamad Mostafa , 2016Description: 133 P. : charts , facsimiles ; 25cmOther title:
  • التطفير و دمج البروتوبلاست لخلايا الأرثروباكتر لتحسين إنتاج الجلوكوز أيزوميريز [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Food Science and Technology Summary: In attempts to construct superior glucose isomerase-producing strains, four strains were screened. Both Arthrobacter sp. and A. missouriensis were proved as the highest producers (16.8 and 15.6 U.ml-1, respectively). UV and Ethyl Methane Sulfonate (EMS) were used for mutagenesis. The mutant EMS 60-25 D generated from Arthrobacter sp. exhibited the highest GI production in this study (49.7 U.ml-1) with 3.2 folds improvement than its wild type. Protoplast fusion technology was successfully. The fusant (C 3-2) exhibited the highest GI synthesis (2.75 folds the wild type). The highest GI- producing fusant C 3-2 was used for the continuous production of HFCS. The maximal glucose conversion was 36% after 12h of isomerization at 60{u00B0}C
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.09.Ph.D.2016.He.M (Browse shelf(Opens below)) Not for loan 01010110070408000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.09.Ph.D.2016.He.M (Browse shelf(Opens below)) 70408.CD Not for loan 01020110070408000

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Food Science and Technology

In attempts to construct superior glucose isomerase-producing strains, four strains were screened. Both Arthrobacter sp. and A. missouriensis were proved as the highest producers (16.8 and 15.6 U.ml-1, respectively). UV and Ethyl Methane Sulfonate (EMS) were used for mutagenesis. The mutant EMS 60-25 D generated from Arthrobacter sp. exhibited the highest GI production in this study (49.7 U.ml-1) with 3.2 folds improvement than its wild type. Protoplast fusion technology was successfully. The fusant (C 3-2) exhibited the highest GI synthesis (2.75 folds the wild type). The highest GI- producing fusant C 3-2 was used for the continuous production of HFCS. The maximal glucose conversion was 36% after 12h of isomerization at 60{u00B0}C

Issued also as CD

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