Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Analytical Chemistry

The present work describes the use of sensors based on molecularly imprinted polymer (MIP) for the electrochemical determination of Itopride hydrochloride (Itoh) and mosapride citrate (MC). To optimize the synthesis of MIPs, first, computational studies were conducted to determine the most suitable functional monomer to be used and the optimum ratio of template to functional monomer in the synthesis, then MIPs with different ratios were synthesized by bulk polymerization following the non-covalent approach. The rebinding of the templates to the synthesized MIPs was evaluated by equilibrium batch rebinding assays. Finally, the MIP with the highest binding capacity was chosen to be incorporated as an additive in sensors for the electrochemical determination of the two drugs. The MIP chosen was the one prepared with the ratio of (template: functional monomer: crosslinker) 1:8:40 for Itoh and MC. For both drugs, methacrylic acid was used as functional monomer and ethylene glycol dimethacrylate or divinyl benzene were used as crosslinkers for Itoh and MC, respectively. The modified sensors were successfully used in the quantification of the drugs in pure solution, pharmaceutical preparations and spiked biological samples (urine and plasma) using the standard additions method with high accuracy and precision

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