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Preparation of monoclonal antibodies against tetanus toxin and evaluation of its neutrelaizing potential / Ahmed Adel Attia Seada ; Supervised Rafik soliman , Nashwa Abdelsalam Ezz_Eldeen

By: Contributor(s): Language: Eng Publication details: Cairo : Ahmed adel attia seada , 2006Description: 99P : ill ; 30cmOther title:
  • تحضير اجسام مضادة وحيدة الفسيلة ضد سم التيتانوس وتقييم قدرتها المنا عية [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Microbiology , Bacteriology , Mycology and Immunology Summary: Preparation of monoclonal antibodies against tetanus toxin and evaluation of its efficacy obtained by using spleen cells (B - Cells) which harvested from well immunized BALB / c mice were used , also myeloma cell lines P3NS / 1 were usedFusion between spleen cells and myeloma cell lines occurred through fusogenic material called PEG 1500 , 40in hundred concentration to produce hybridomas which had the characteristic of both parents the ability to grow indefinitely from myeloma and the ability to produce of specific antibody from the B - cellsIn this study we succeed to produce five hybridomas producing monoclonal antibodies against tetanus toxin and also succeed to be cloned into 5 stable clones producing monoclonal antibodies against tetanus toxin
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2006.Ah.P. (Browse shelf(Opens below)) Not for loan 01010110045976000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2006.Ah.P. (Browse shelf(Opens below)) Not for loan 01020110045976000

Thesis (M.Sc.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Microbiology , Bacteriology , Mycology and Immunology

Preparation of monoclonal antibodies against tetanus toxin and evaluation of its efficacy obtained by using spleen cells (B - Cells) which harvested from well immunized BALB / c mice were used , also myeloma cell lines P3NS / 1 were usedFusion between spleen cells and myeloma cell lines occurred through fusogenic material called PEG 1500 , 40in hundred concentration to produce hybridomas which had the characteristic of both parents the ability to grow indefinitely from myeloma and the ability to produce of specific antibody from the B - cellsIn this study we succeed to produce five hybridomas producing monoclonal antibodies against tetanus toxin and also succeed to be cloned into 5 stable clones producing monoclonal antibodies against tetanus toxin

Issued also as CD

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