Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy

Zingiber officinale commonly called ginger is being used in Arabian folk medicine for treatment of different diseases including improvement of memory impairment. The present study was designed to evaluate the role of ginger in treatment of alzheimer{u2019}s disease in-vitro and in-vivo. The phytochemical study was carried out using different chromatographic methods including GC/MS analysis of the volatile constituents of ginger essential oils (hydro-distilled and commercial) and phytochemical investigation of ginger methanolic extract using semi-preparative HPLC. Ginger methanolic extract and ginger essential oils (hydro-distilled and commercial) were tested for their antioxidant activity in-vitro against diphenylpicrylhydrazyl (DPPH) free radical and the inhibition activity against acetylcholinesterase enzyme (AChE) was tested using a multi-well plate Ellman{u2019}s assay. In addition, the methanolic extract and the commercial ginger oil were studied in-vivo using Alzheimer{u2019}s disease model induced in rats using oral AlCl3. Also, the antioxidant activity in-vitro of the isolated compounds from ginger methanolic extract was evaluated using DPPH free radical scavenging assay, superoxide radical scavenging activity assay, hydrogen peroxide scavenging activity assay and reducing power assay as well as the inhibition activity against acetylcholinesterase enzyme (AChE) was evaluated using a multi-well plate Ellman{u2019}s assay. Results showed that both essential oils participated in 25 common volatile components

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