header
Local cover image
Local cover image
Image from OpenLibrary

Assessment of the trans-differentiation potential of bone marrow and umbilical cord blood stem cells in generating insulin producing cells / Sara Mohamed Abdelkhalek Abdelrazek ; Supervised Mahmoud Ahmed Amer , Amira Mohamed Raafat , Abeer Ahmed Bahnassy

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Sara Mohamed Abdelkhalek Abdelrazek , 2016Description: 137 P. : photographs ; 25cmOther title:
  • {uFE97}{uFED8}{uFEF4}{uFEF4}{uFEE2} إ{uئإإ٣}{uئإؤأ}{uئإ٨إ}{uئإإ٧}{uئإئ٤}{uئإ٩٤} {uئإ٩٧}{uئإإ٤}{uئإ٨إ}{uئإئ٣}{uئإآ٠} ا{uئإؤئ}{uئإء٨}{uئإئأ}{uئإئ٣}{uئإ٨إ} {uئإإ٣}{uئإإ٦} {uئإإ٧}{uئإء٨}{uئإ٨إ}ع ا{uئإؤئ}{uئإأأ}{uئإأ٨}{uئإإ٢} و ا{uئإؤئ}{uئإء٨}{uئإئأ}{uئإئ٣}{uئإ٨إ} ا{uئإؤئ}{uئإء٠}{uئإءأ}{uئإأآ}{uئإئ٤}{uئإ٩٤} {uئإؤئ}{uئإءء}م ا{uئإؤئ}{uئإء٤}{uئإ٩٢}{uئإؤإ} ا{uئإؤئ}{uئإآ٤}{uئإءإ}ى فى {uئإ٩٧}{uئإإإ}{uئإؤئ}{uئإئ٤}{uئإءء} {uئإء٧}{uئإئأ}{uئإئ٣}{uئإ٨إ} إ{uئإإ٧}{uئإ٩٨}{uئإ٨إ}ج ا{uئإئ٧}{uئإإ٧}{uئإآ٤}{uئإإإ}{uئإؤئ}{uئإئ٤}{uئإإ٦} [Added title page title]
Subject(s): Online resources: Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology Summary: This study was carried out to assess the feasibility of generating insulin-producing cells from progenitor cells obtained from the bone marrow (BM) and cord blood (CB) and compare the trans-differentiation potential of the two studied sources (BM versus CB cells). I- In this study, 20 samples of umbilical cord blood were obtained, isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in-vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology insulin-producing cells appeared. II- Twenty samples of rat bone marrow were obtained; isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology, and became insulin-producing cells appeared. III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of GAPDH, Insulin genes were assessed using RT-PCR technique
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Home library Call number Copy number Status Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2016.Sa.A (Browse shelf(Opens below)) Not for loan 01010110073932000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2016.Sa.A (Browse shelf(Opens below)) 73932.CD Not for loan 01020110073932000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

This study was carried out to assess the feasibility of generating insulin-producing cells from progenitor cells obtained from the bone marrow (BM) and cord blood (CB) and compare the trans-differentiation potential of the two studied sources (BM versus CB cells). I- In this study, 20 samples of umbilical cord blood were obtained, isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in-vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology insulin-producing cells appeared. II- Twenty samples of rat bone marrow were obtained; isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology, and became insulin-producing cells appeared. III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of GAPDH, Insulin genes were assessed using RT-PCR technique

Issued also as CD

There are no comments on this title.

to post a comment.

Click on an image to view it in the image viewer

Local cover image