Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology

Objectives: The aim of the present study is to investigate the effects of the green tea, barley anddoxorubicin and their combination on some changes produced by experimentally induced cancer by N-dimethylnitrosamine (DMN) in rats. Methods: cancerwasinduced in rats byadministration of DMN (25 mg/kg) orally daily for 6 weeks. Group1: given saline orally and servedas normal control. Group2: received DMN for 6 weeks and served as cancer control. Group3-4: given green tea extract (1 g/ kg) and barley extract (625 mg/ kg), orally for 4 weeks. Group 5-8: all ratsgiven DMNfor 6 weeks then treated with green tea, barley, doxorubicin and their combination of green tea,barley and doxorubicin for 4 weeks starting from the second week of DMN. Results: DMN provoked kidney and liver apoptosis as evidenced byelevation of kidney and liver functions, decrease in serum calcium level, elevation of tumor marker alpha-fetoprotein(AFP), ferritin and lactate dehydrogenase (LDH). DMN group had higher kidney nitric oxide(NO) and malondialdehyde (MDA) contents and lower superoxide dismutase (SOD), as well as it inducedinflammation, apoptosis and caspase-3 expression. Green, barley and their combination improved thebiochemical and histopathological changes in kidney and liver induced by DMN. Conclusion: This study indicates thetherapeutic effect of green tea, barley against DMN-induced kidney and liver apoptosis. Interestingly, ourresults exhibited that doxorubicin conjugated with green tea and barley increased renal and hepatic protectionactivity and may decrease systemic toxicity of doxorubicin when administered in high dose

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