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Effective diagnosis of human Toxoplasmosis using superparamagnetic nanoparticles by ELISA technique / Amal Mahfouz Abdelreheim ; Supervised Azza Mohamed Elamir , Noha Ahmed Mahana , Alyaa Ahmed Farid

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Amal Mahfouz Abdelreheim , 2016Description: 112 P. : charts , facsimiles ; 25cmOther title:
  • التشخيص الفعال لمرض التكسوبلازما في الإنسان بإستخدام الجزيئات النانونيه لأشباه المغناطيس الفائقة بتقنية الأليزا [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology Summary: Parasitic diseases have a great impact in human health. The gold standard for the diagnosis of the majority of parasitic infections is still conventional microscopy, which presents important limitations in terms of sensitivity and specificity and commonly requires highly trained technicians. More accurate technique, gold nanoparticles (AuNPs) conjugated with anti-Toxoplasma gondii (T. gondii) polyclonal antibody (pAb) was evaluated to increase the sensitivity and specificity of antigen detection assay (sandwich-ELISA and dot-ELISA) for diagnosing human T. gondii infection. In the present study, T. gondii SAG2 antigen was purified (37 KDa) and anti-SAG2 IgG pAb was prepared in rabbits. T. gondii SAG2 antigen was early detected in control and patient sera using nanogold sandwich and nanogold dot-ELISA. Two different nanogold ELISA showed a higher significant sensitivity (94% and 98%) and specificity (96% and 96%) in comparison to normal sandwich and dot-ELISA which showed 92% and 96% sensitivity and 90% and 94% specificity, respectively. From all recorded data and with consideration to sandwich and dot-ELISA assays, nanogold-sandwich and nanogold dot-ELISA assay have a promising and important value as an applicable, fast and accurate diagnostic technique for toxoplasmosis
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.Ph.D.2016.Am.E (Browse shelf(Opens below)) Not for loan 01010110074551000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.Ph.D.2016.Am.E (Browse shelf(Opens below)) 74551.CD Not for loan 01020110074551000

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology

Parasitic diseases have a great impact in human health. The gold standard for the diagnosis of the majority of parasitic infections is still conventional microscopy, which presents important limitations in terms of sensitivity and specificity and commonly requires highly trained technicians. More accurate technique, gold nanoparticles (AuNPs) conjugated with anti-Toxoplasma gondii (T. gondii) polyclonal antibody (pAb) was evaluated to increase the sensitivity and specificity of antigen detection assay (sandwich-ELISA and dot-ELISA) for diagnosing human T. gondii infection. In the present study, T. gondii SAG2 antigen was purified (37 KDa) and anti-SAG2 IgG pAb was prepared in rabbits. T. gondii SAG2 antigen was early detected in control and patient sera using nanogold sandwich and nanogold dot-ELISA. Two different nanogold ELISA showed a higher significant sensitivity (94% and 98%) and specificity (96% and 96%) in comparison to normal sandwich and dot-ELISA which showed 92% and 96% sensitivity and 90% and 94% specificity, respectively. From all recorded data and with consideration to sandwich and dot-ELISA assays, nanogold-sandwich and nanogold dot-ELISA assay have a promising and important value as an applicable, fast and accurate diagnostic technique for toxoplasmosis

Issued also as CD

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