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Algal-based expression of hemagglutinin (HA2) protein of avian influenza virus (AIV) : A step towards a universal vaccine / Mahmoud Mohamed Elsayed Bayoumi ; Supervised Ausama Abdelraouf A. Yousif , Haitham Mohamed Mohamed Amer

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mahmoud Mohamed Elsayed Bayoumi , 2017Description: 189 P. : charts , facsimiles ; 25cmOther title:
  • التعبير في الطحالب لبروتين التلزن الدموى{u٠٦ئ٢} لفيروس إنفلونزا الطيور إتش٥إن ١ : خطوة تجاه لقاح شامل [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology Summary: Green biotechnology is the future of biopharmaceuticals production. The use of algae for the production of biopharmaceuticals is of particular interest in vaccine-based control programs. An attempt was made to express avian influenza virus (AIV) immunogens in algae because the virus is a serious economic and public health threat. A commercial eukaryotic expression system was modified to allow expression of AIV H5N1 hemagglutinin subunit 2 (HA2) in the microalga Chlamydomonas reinhardtii (C. reinhardtii). Codon-optimized AIV H5N1 HA2 sequences were synthesized and cloned (coHA2) into a cloning vector pUC-57. coHA2 sequences were then transferred to algal expression vector pChlamy_3/D- TOPO® then to C. reinhardtii strain cc-125. Proper nuclear integration was achieved in 16% of the screened transformants that survived selection in hygromycin-containing TAP media. coHA2 mRNA transcription was confirmed using RT-PCR, GFP expression using Confocal microscopy, AIV HA2 expression was confirmed using western blot analysis utilizing AIV H5N2 chicken antisera. These results warrant further investigation into immunogenic potential of the algae-expressed HA2
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2017.Ma.A (Browse shelf(Opens below)) Not for loan 01010110074344000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2017.Ma.A (Browse shelf(Opens below)) 74344.CD Not for loan 01020110074344000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

Green biotechnology is the future of biopharmaceuticals production. The use of algae for the production of biopharmaceuticals is of particular interest in vaccine-based control programs. An attempt was made to express avian influenza virus (AIV) immunogens in algae because the virus is a serious economic and public health threat. A commercial eukaryotic expression system was modified to allow expression of AIV H5N1 hemagglutinin subunit 2 (HA2) in the microalga Chlamydomonas reinhardtii (C. reinhardtii). Codon-optimized AIV H5N1 HA2 sequences were synthesized and cloned (coHA2) into a cloning vector pUC-57. coHA2 sequences were then transferred to algal expression vector pChlamy_3/D- TOPO® then to C. reinhardtii strain cc-125. Proper nuclear integration was achieved in 16% of the screened transformants that survived selection in hygromycin-containing TAP media. coHA2 mRNA transcription was confirmed using RT-PCR, GFP expression using Confocal microscopy, AIV HA2 expression was confirmed using western blot analysis utilizing AIV H5N2 chicken antisera. These results warrant further investigation into immunogenic potential of the algae-expressed HA2

Issued also as CD

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