Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

Cancer is a genetic disease it is caused by changes to genes that control the way of cells function, especially cell growth and division. Selection of cancer treatment is affected by various factors including features of the tumor and patients conditions. Many natural products have anti-tumor effects against different types of cancer, where they have different mechanisms of action including cell growth suppression, modulation of cell differentiation and induction of apoptosis. Moringa oleifera (L) is a commonly used in phytotherapy and herbal medicine that have an anti-inflammatory, anti-spasmodic, anti-hypertensive, anti-oxidant, anti-pyretic, anti-ulcer, anti-epileptic, diuretic, cholesterol lowering, renal, anti-diabetic effects and anti-tumor effects. In the present study; Moringa oleifera (L) leaves aqua{u2019}s extract were evaluated for its cytotoxic potentiality against three tumor cell lines; (HepG2, Caco2 and Mcf7). Cytoxic effects of M.O L-WX by MTT assay revealed remarkable dose dependent anti-cancer activity on Caco2 and Mcf7 while lower anticancer activity on HepG2. The IC value were 4 mg /mL and 9 mg /mL fo 50 CaCo2 and McF7 cancer cell lines respectively while it was 19 mg /mL for Hepg2 cells. The molecular mechanism based study revealed an apoptotic effect; this as evaluated by the significant increase in expression of apoptotic molecules FASL of Caco2 and Mcf7 cell lines (p-value=0.001 and 0.005 respectively) when compared to the control of each cell and also the significant increase in the activity of the caspase8 on Caco2 and Mcf7 (p-value =0.029and 0.007 respectively ), caspase9 (p-value=0.01 and 0.0001 respec-tively), and caspase3 activity (p-value=0.008and 0.004 respectively) when compared to its control

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