Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Physiology

Objectives: Test the possible renoprotective effects of angiotensin converting enzyme inhibitor and thiamine in type 2 diabetic kidneys . Methods: A total of 50 adult albino rats were used in this study weighing 150-200 grams. The animals were housed in wire mesh cages at room temperature, with normal light/dark cycle. They were fed the commercial rat chow diet and had free access to water. These rats were divided into the following groups: : Group 1 : Control group, Group 2 : Type 2 diabetic rats, Group 3 : Type 2 diabetic nephropathy rats, Group 4 : Lisinopril-treated type 2 diabetic nephropathy rats, Group 5 : Thiamine mononitrate-treated type 2 diabetic nephropathy rats. Blood samples were collected for measurement of fasting blood glucose, fasting blood insulin, blood urea and blood albumin creatinine ratio . Homeostasis model assessment of insulin resistance was calculated. Urine samples were collected for measurement of urinary albumin excretion. Systolic blood pressure and diastolic blood pressure were also measured . Nuclear factor kappa B gene expression was estimated . Results: Treatment with angiotensin converting enzyme inhibitor reduced fasting blood glucose, fasting blood insulin, blood urea, urinary albumin excretion, nuclear factor kappa B gene expression, systolic blood pressure and diastolic blood pressure. Treatment with thiamine mononitrate reduced fasting blood glucose, blood urea, urinary albumin excretion and systolic blood pressure. Conclusion: The use of angiotensin converting enzyme inhibitor and thiamine in the treatment of kidney disease with type 2 diabetes significantly offered renal protection and improved the diabetic condition and may postpone the onset of renal injury in a type 2 diabetic rats

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