Biological study on the effect of transforming growth factors on hepatic somatic stem cells propagated ex vivo / Mohammed Zaghloul Mohammed ; Supervised Motawa E. Elhussieni , Alaa Eldean Ismael A. Almottleb , Ahmad H. Elhabashy
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- دراسة بيولوجية على تأثيرعوامل النموعلى الخلايا الجذعية الجسدية الكبدية المعزولة من مرضى سرطان الكبد [Added title page title]
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قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.19.02.Ph.D.2018.Mo.B (Browse shelf(Opens below)) | Not for loan | 01010110075789000 | ||
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مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.19.02.Ph.D.2018.Mo.B (Browse shelf(Opens below)) | 75789.CD | Not for loan | 01020110075789000 |
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Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology
Objective: This study had attempted to study the effect of TGF-Ý1 on hepatic somatic stem cells (HSSC) in vitro, since it could play an active role in the process of hepatocarcinogenesis and cancer metastases. We aimed to determine the HCC-specific molecular mechanisms mediating TGF-Ý1 dysregulation to a driver of tumor progression and characterize the molecular network between TGF-Ý1 and c-KIT signaling. Methods: Cancer stem cells were isolated from Hepatocellular carcinoma patients of different grades, and from age and sex matched healthy controls from safety margin and R N A isolation was done to get PCR product for mutations detection. Results: The results of this study showed that by using monoclonal characterization CD44 and CD90 for non-malignant liver tissue origin cells there are significant decreasing in CD40+CD90+ %by increasing TGF Ý1 concentration (P value <0.001). while for malignant liver tissue origin cells there are significant decreasing in CD40+CD90+ % only by using TGF Ý1 at concentration2.5 and 5 ng/dl P value <0.001
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