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Important virulence factors and related genes in uropathogenic E. coli and their relation to fluoroquinolone resistance / Hanaa Fathy Aly Abdelatty ; Supervised Magda Ibrahim Mohamed Ayoub , Noha Mahmoud Abdelrahim Gohar

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hanaa Fathy Aly Abdelatty , 2018Description: 118 P. : charts , facsimiles ; 25cmOther title:
  • عوامل الضراوة المهمة وجيناتها فى العصيات القولونية المسببة لعدوى المجارى البولية وعلاقتها بمقاومة المضاد الحيوى فلوروكينولون [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology Summary: Escherichia coli(E. coli) is the predominant pathogen causing urinary tract infection. E. coli expresses a wide spectrum of virulence factors that enable it to cause the disease. The present study was conducted on 50 E. coli isolates to assess some important phenotypic virulence factors of E. coli: haemolytic activity, cytotoxic activity and haemagglutination and their relations to the following virulence genes: hlyA, cnf-1 and papC, by using multiplex PCR. Fluoroquinolones susceptibility was done using disk diffusion method.The results showed that 30% of the isolates were Ý- haemolytic, 18% of the isolates showed haemagglutination with human RBCs, with higher prevalence for MRHA (12%) than MSHA (6%), and 92% of the isolates showed morphological changes, detachment and lysis in HEp-2 cells. Multiplex PCR showed that the most prevalent gene was cnf-1(30%), while 26% of isolates were positive for hlyA and 26% of isolates were positive for papC. Only one UPEC isolate showed the simultaneous presence of papC, cnf-1 and hlyA while 10 isolates were harbouring hlyA and cnf-1, 2 isolates were harbouring cnf-1 and papC genes and 2 isolates were harbouring hlyA and papC simultaneously. We found that hlyA gene was significantly detected in the Ý- haemolytic than non- haemolytic isolates. As regard fluoroquinolones susceptibility, 60% of the isolates were resistant to the used fluoroquinolones with no significant difference between resistant and susceptible isolates as regard virulence factors and genes
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.Ph.D.2018.Ha.I (Browse shelf(Opens below)) Not for loan 01010110076567000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.Ph.D.2018.Ha.I (Browse shelf(Opens below)) 76567.CD Not for loan 01020110076567000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

Escherichia coli(E. coli) is the predominant pathogen causing urinary tract infection. E. coli expresses a wide spectrum of virulence factors that enable it to cause the disease. The present study was conducted on 50 E. coli isolates to assess some important phenotypic virulence factors of E. coli: haemolytic activity, cytotoxic activity and haemagglutination and their relations to the following virulence genes: hlyA, cnf-1 and papC, by using multiplex PCR. Fluoroquinolones susceptibility was done using disk diffusion method.The results showed that 30% of the isolates were Ý- haemolytic, 18% of the isolates showed haemagglutination with human RBCs, with higher prevalence for MRHA (12%) than MSHA (6%), and 92% of the isolates showed morphological changes, detachment and lysis in HEp-2 cells. Multiplex PCR showed that the most prevalent gene was cnf-1(30%), while 26% of isolates were positive for hlyA and 26% of isolates were positive for papC. Only one UPEC isolate showed the simultaneous presence of papC, cnf-1 and hlyA while 10 isolates were harbouring hlyA and cnf-1, 2 isolates were harbouring cnf-1 and papC genes and 2 isolates were harbouring hlyA and papC simultaneously. We found that hlyA gene was significantly detected in the Ý- haemolytic than non- haemolytic isolates. As regard fluoroquinolones susceptibility, 60% of the isolates were resistant to the used fluoroquinolones with no significant difference between resistant and susceptible isolates as regard virulence factors and genes

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