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Relative telomeric length and polymorphism in telomere maintenance gene telomerase reverse transcriptase in hepatocellular carcinoma patients / Asmaa Esmail Ali Nasr Eldin ; Supervised Enas Hamdy Mahmoud , Ahmed Ameen Emara Hekel , Alaa Afif Abdelrauof

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Asmaa Esmail Ali Nasr Eldin , 2018Description: 112 P. : charts , facsimiles ; 25cmOther title:
  • النسبة الطولية لتلومير وتعدد اشكال في عكس التلموريز الناسخ في الجينات في مرضي سرطان الكبد [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Background: Telomere length and telomerase activity are crucial for cancer initiation and the survival of tumors. Telomere length is maintained by telomerase in 90% of human cancers,while 10% of cancers use an alternative mechanism of telomere lengthening termed (ALT).Another mechanism for Cancer cells is through activating or upregulating the normally silent human TERT gene (hTERT) that encodes telomerase Objectives:The aim of the present study wasto assess the role of telomeric length in the development of hepatocellular carcinoma(HCC) and to study the effect of 2SNPs in TERT gene rs13167280, rs2736098 on telomere length Subjects and Methods: the study was conducted on a total number of 100 patients,subdivided into two groups HCC and HCV groups.telomeric length was measured using quantitative real time PCR(sybergreen),SNP were measuring using Taqman probe on step{u2013}one real time PCRResults:We found thatthe patients with HCC had a significant short relative telomere length(RTL)with median level 0.15 compared to the median of HCV group which was 1.03 with the p-value <0.001.The performance of Relative telomere length was detected using ROC Curve. At the cut-off value of 0.7, RTL showed sensitivity 92 %, specificity 94%, diagnostic accuracy 93% and the area under the curve (AUC) were 0.917, and there was no statistical significant difference observed on comparing the two SNPs in HCC and HCV groups Conclusions: the median value of RTL in HCC patients was short compared to HCV patients, so the relative telomeric length could be used as a non invasive biomarker for HCC
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2018.As.R (Browse shelf(Opens below)) Not for loan 01010110076653000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2018.As.R (Browse shelf(Opens below)) 76653.CD Not for loan 01020110076653000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Telomere length and telomerase activity are crucial for cancer initiation and the survival of tumors. Telomere length is maintained by telomerase in 90% of human cancers,while 10% of cancers use an alternative mechanism of telomere lengthening termed (ALT).Another mechanism for Cancer cells is through activating or upregulating the normally silent human TERT gene (hTERT) that encodes telomerase Objectives:The aim of the present study wasto assess the role of telomeric length in the development of hepatocellular carcinoma(HCC) and to study the effect of 2SNPs in TERT gene rs13167280, rs2736098 on telomere length Subjects and Methods: the study was conducted on a total number of 100 patients,subdivided into two groups HCC and HCV groups.telomeric length was measured using quantitative real time PCR(sybergreen),SNP were measuring using Taqman probe on step{u2013}one real time PCRResults:We found thatthe patients with HCC had a significant short relative telomere length(RTL)with median level 0.15 compared to the median of HCV group which was 1.03 with the p-value <0.001.The performance of Relative telomere length was detected using ROC Curve. At the cut-off value of 0.7, RTL showed sensitivity 92 %, specificity 94%, diagnostic accuracy 93% and the area under the curve (AUC) were 0.917, and there was no statistical significant difference observed on comparing the two SNPs in HCC and HCV groups Conclusions: the median value of RTL in HCC patients was short compared to HCV patients, so the relative telomeric length could be used as a non invasive biomarker for HCC

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