Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine- Department of Food Hygiene and Control

Chitosan is a natural bioactive polysaccharide, derived from the deacetylation of chitin with intrinsic antimicrobial activity and can be recognized as a natural alternative to synthesized antimicrobial for extending perishable food shelf life. For exploring the effect of chitosan and lactic acid and their combination on extending the shelf life of chicken breast fillets at refrigerator storage and decontamination of Salmonella Typhimurium and E.coli,chicken breast fillets were treated with chitosan 0.5% ,chitosan 1% , chitosan 2% and lactic acid 1% solutions beside their combination and stored at 4 o C. Treated samples were examined for sensory attributes, deterioration criteria and bacterial load at regular intervals. Samples treated with chitosan 0.5% didn{u2019}t significantly (P<0.05) differ from the control, meanwhile treated samples withchitosan 1%, 2% , lactic acid 1% and combination between chitosan 2% and Lactic acid 1% extended chicken fillet shelf life by three days more than the control. Although using of lactic acid 1% reduce the fillet bacterial load as chitosan 2% treated samples, however the sensory properties of treated samples with chitosan 2% were significantly higher than lactic acid 1%treated samples, as lactic acid 1% were showing significant reduction in their sensory attributes

Issued also as CD