header
Local cover image
Local cover image
Image from OpenLibrary

Study of MicroRNA 196a and 499 polymorphisms in hepatocellular carcinoma patients / Asmaa Mohamed Fteah Abdelaziz ; Supervised Nehad Ahmed Mosaad , Mona Mohamed Hassan , Asmaa Ismail Ahmed

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Asmaa Mohamed Fteah Abdelaziz , 2018Description: 170 P. : charts ; 25cmOther title:
  • فى مرضى سرطان الكبد MICRORNA 196A and 499 دراسة تأثير التعددات الشكلية لـــ [Added title page title]
Subject(s): Online resources: Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Background: Hepatocellular carcinoma (HCC) is the commonest primary tumor of the liver. MicroRNAs (miRNAs) can generate different functions for regulating RNA protein levels and balancing abnormalities. Single nucleotide polymorphisms (SNPs) in miRNAs were reported to increase patient susceptibility to cancer development and affect prognosis and survival. Objective: To evaluate MiRNA 196a2 (rs11614913) C>T and MiRNA 499 (rs3746444) A>G as potential biomarkers for HCV-related HCC development in Egyptian patients. Subjects and methods: A case-control study included 75 HCV-related HCC patients, 75 cirrhotic patients on top of HCV infection and 75 healthy controls. Genotyping of the candidate SNPs was performed by Real Time PCR. Liver and kidney function tests, hepatitis B and C markers and AFP were performed. Results: There was a significant difference in miRNA 499 (rs3746444) genotypes frequency between the three studied groups (p=0.009) and the combined microRNA 499 (AA+AG) genotypes were significantly higher in HCC cases than in cirrhosis and normal control groups (p=0.005). The frequency of the G allele was significantly lower in HCC cases than other groups (p=0.024) and significantly lower in HCC cases than normal control group (p=0.006) and [OR (95%CI) = 0.501 (0.304-0.825)]. Median AST, ALT, ALP, total bilirubin and direct bilirubin levels were found to be significantly lower in patients with GG genotype than those with (AA+AG) genotypes with (p = 0.002, 0.036, 0.006, 0.021 and 0.01) respectively. For miRNA 196a2 (rs11614913) C>T polymorphisms, no significant association was found with the risk for HCC development
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Home library Call number Copy number Status Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2018.As.S (Browse shelf(Opens below)) Not for loan 01010110077877000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2018.As.S (Browse shelf(Opens below)) 77877.CD Not for loan 01020110077877000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Hepatocellular carcinoma (HCC) is the commonest primary tumor of the liver. MicroRNAs (miRNAs) can generate different functions for regulating RNA protein levels and balancing abnormalities. Single nucleotide polymorphisms (SNPs) in miRNAs were reported to increase patient susceptibility to cancer development and affect prognosis and survival. Objective: To evaluate MiRNA 196a2 (rs11614913) C>T and MiRNA 499 (rs3746444) A>G as potential biomarkers for HCV-related HCC development in Egyptian patients. Subjects and methods: A case-control study included 75 HCV-related HCC patients, 75 cirrhotic patients on top of HCV infection and 75 healthy controls. Genotyping of the candidate SNPs was performed by Real Time PCR. Liver and kidney function tests, hepatitis B and C markers and AFP were performed. Results: There was a significant difference in miRNA 499 (rs3746444) genotypes frequency between the three studied groups (p=0.009) and the combined microRNA 499 (AA+AG) genotypes were significantly higher in HCC cases than in cirrhosis and normal control groups (p=0.005). The frequency of the G allele was significantly lower in HCC cases than other groups (p=0.024) and significantly lower in HCC cases than normal control group (p=0.006) and [OR (95%CI) = 0.501 (0.304-0.825)]. Median AST, ALT, ALP, total bilirubin and direct bilirubin levels were found to be significantly lower in patients with GG genotype than those with (AA+AG) genotypes with (p = 0.002, 0.036, 0.006, 0.021 and 0.01) respectively. For miRNA 196a2 (rs11614913) C>T polymorphisms, no significant association was found with the risk for HCC development

Issued also as CD

There are no comments on this title.

to post a comment.

Click on an image to view it in the image viewer

Local cover image