Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

Introduction:Preeclampsia(PE)isapregnancy-specifichypertensivediseasewhose etiopathogenesis remains unclear. Objectives: This study was designed to assess association between PE and 3 single nucleotide polymorphisms (SNPs): ENG (G/A) rs11792480, TGFÝR1 (A/C) rs10739778 and TGFÝR2 (G/A) rs6550005, beside the circulating level of soluble endoglin (sENG), oxidative stress biomarkers and nitric oxide (NO) in Egyptian women. Methods: The study included 75 preeclamptic women stratified into 4 clinical subgroups and 50 normotensive pregnant women. Genotyping was performed by real time polymerase chain reaction-TaqMan allelic discrimination. Results: Preeclamptic women showed significantly increased sENG and malondialdehyde (MDA) levels, decreased total antioxidant capacity (TAC), endothelial nitric oxide synthase (eNOS) and NO levels, without change in transforming growth factor beta 1 (TGFÝ1) level versus controls. Moreover, sENG was significantly higher in severe than mild PE and in early- than late-onset PE. Higher MDA and lower TAC and NO levels were observed in severe than mild PE. ENG (G/A) and TGFÝR2 (G/A) showed no association with PE. However, CC genotype of TGFÝR1 (A/C) was more frequent in controls than either PE, early-onset or severe PE revealing a reduced PE risk in CC genotype versus AA or AA+AC. Importantly, patients carrying AA genotype had higher systolic blood pressure (SBP) and MDA with lower TAC level, gestational age (GA) at delivery and birth weight than those carrying CC genotype

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