Thesis (M.Sc.) - Cairo University -Faculty of Medicine - Department of Pharmacology

Introduction: The most common types of IBD are Crohn{u2019}s disease (CD) and ulcerative colitis (UC). So far, the main therapeutics broadly available to treat IBD are based on suppressing the immune response. But these therapies are unable to reset the intestinal homeostasis and do not directly treat the underlying cause of the chronic inflammation Materials and methods Seventy mice were included in this study and they were divided into 7 group. After fasting for 24 hours and under isoflurane 2% anaethesia, colonic lavage with normal saline was done using a polypropylene trocar cannula inserted through the rectum. Group 1 is a control negative group, while the other six groups received 0.1ml of acetic acid 4% intrarectally that are left for 15 seconds in contact with the colonic tissue to induce colitis followed by colonic lavage with saline. Twenty four hours later group 1(control negative) and group 2(control positive) were left untreated while the other five groups were treated as follow: -Group 3a: Mice in this group received Liraglutide at a starting dose of 0.3mg/kg/day S.C injection, increased to 0.6 mg/kg/day on day 2 of treatment and then 1mg/kg/d from day 3 to day 10 by S.C injection once daily. -Group 3b : Mice in this group received Sitagliptin 100 mg/kg/d orally by oral gavage once daily for 10 days. -Group 3c: Mice in this group received Sulfasalazine in a dose of 100 mg/kg/d orally by oral gavage once daily for 10 days.-Group 3d: Mice in this group received a combination of both drugs (Liraglutide at a starting dose of 0.3 mg/kg/day S.C injection, increased to 0.6 mg/kg/day on day 2 of treatment and then 1mg/kg/d from day 3 to day 10 by S.C injection once daily and Sulfasalazine in a dose of 100 mg/kg/d orally by oral gavage) for 10 days. -Group 3e: Mice in this group received a combination of both drugs (Sitagliptin 100 mg/kg/d orally by oral gavage once daily and Sulfasalazine in a dose of 100 mg/kg/d orally by oral gavage) for 10 days

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