Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Hepatocellular carcinoma is the fifth most common form of cancer worldwide and the second most common cause of cancer-related deaths. There are multiple etiologic factors affecting HCC. Chronic hepatitis B and C with associated liver cirrhosis represent major risk factors for HCC development, being implicated in more than 70% of HCC cases worldwide. Many studies have suggested the associations between HCC cancer risk and SNPs in selected genes. Aberrant WISP1 expression may be associated with carcinogenesis. Aim of work: The current study aimed at detecting rs2977530 and rs62514004 polymorphisms of WISP1 gene and susceptibility to HCC in Egyptians. Subjects and Methods: The current case-control study was conducted on 90 subjects classified into two groups: HCC group included 60 patients attending the HCC clinic at Kasr al Ainy Hospitals, compared to a control group including 30 apparently healthy subjects. Genotyping was performed by using Real time-PCR (TaqMan probes). Results: The study showed that no statistically significant association between the WISP1 (rs2977530 and rs62514004) SNPs and the susceptibility to HCC. The laboratory findings in this study revealed statistically significant association between rs62514004 genotype frequencies and two of the laboratory findings: INR and prothrombin time (P value 0.019 and 0.007 with OR 3.953 and 5.844, respectively). While there was no statistically significant association between rs2977530 and HCC risk factors and clinicolaboratory findings

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