header
Image from OpenLibrary

Evaluation of interleukin 17 gene expression in relation to the therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) in CCl₄- induced rat liver fibrosis / Shimaa Farouk Abdelrahman Ali ; Supervised Mohamed Akmal A. Elghor , Salwa Farouk Sabet , Fatma A. Abu Zahra

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Shimaa Farouk Abdelrahman Ali , 2019Description: 181 P. : charts ; 25cmOther title:
  • CCl₄ تقييم التعبير الجينى للانترليوكين 17 و علاقته بالتأثير العلاجى للخلايا الجذعية الوسيطة المشتقة من نخاع العظم فى فئران مستحثه للتليف الكبدى بواسطة رابع كلوريد الكربون [Added title page title]
Subject(s): Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology Summary: Therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) was reported in several animal models of liver fibrosis. Interleukin (IL) 17A, IL6 and STAT3 have been described to play crucial roles in chronic liver injury. However, the modulatory effect of MSCs on these markers was controversial in different diseases. The immunomodulatory role of BM-MSCs on IL17A/IL6/STAT3 was not fully elucidated in liver fibrosis. In the present study, we evaluated the capacity of the BM-MSCs in the modulation of cytokines milieu and signal transducers, based on unique inflammatory genes Il17a and Il17f and their receptors Il17rc and their effect on the IL6/STAT3 pathway in CCl₄- induced liver fibrosis in rats. A single dose of BM-MSCs was administered to the group with induced liver fibrosis, and the genes and proteins of interest were evaluated along six weeks after treatment. Our results showed a significant downregulation of Il17a, Il17ra, il17f and Il17rc genes. In accordance, BM-MSCs administration declined IL17, IL2 and IL6 serum proteins and downregulated IL17A and IL17RA proteins in liver tissue. Interestingly, BM-MSCs downregulated both Stat3 mRNA expression and p-STAT3, while Stat5a gene was downregulated and p-STAT5 protein was elevated. Also P-SMAD3 and TGFÝR2 proteins were downregulated in response to BM-MSCs treatment. Collectively, we suggest that BM-MSCs might play an immunomodulatory role in the treatment of liver fibrosis through downregulation of IL17A affecting IL6/STAT3 signaling pathway
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.Ph.D.2019.Sh.E (Browse shelf(Opens below)) Not for loan 01010110079958000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.Ph.D.2019.Sh.E (Browse shelf(Opens below)) 79958.CD Not for loan 01020110079958000
Browsing المكتبة المركزبة الجديدة - جامعة القاهرة shelves Close shelf browser (Hides shelf browser)
No cover image available
No cover image available
No cover image available
No cover image available
No cover image available
No cover image available
No cover image available
Cai01.12.21.Ph.D.2019.Sa.D Development of a field applicableimmunostrip assay for rapid detection of circulating schistosoma antigen based on monoclonal antibodies labelled nanoparticles / Cai01.12.21.Ph.D.2019.Sa.D Development of a field applicableimmunostrip assay for rapid detection of circulating schistosoma antigen based on monoclonal antibodies labelled nanoparticles / Cai01.12.21.Ph.D.2019.Sh.E Evaluation of interleukin 17 gene expression in relation to the therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) in CCl₄- induced rat liver fibrosis / Cai01.12.21.Ph.D.2019.Sh.E Evaluation of interleukin 17 gene expression in relation to the therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) in CCl₄- induced rat liver fibrosis / Cai01.12.21.Ph.D.2019.Sh.P Potential genotoxicity and anti-tumor activity in tumor-bearing mice treated with zinc oxide nanoparticles and N-acetyl cysteine / Cai01.12.21.Ph.D.2019.Sh.P Potential genotoxicity and anti-tumor activity in tumor-bearing mice treated with zinc oxide nanoparticles and N-acetyl cysteine / Cai01.12.21.Ph.D.2019.Wa.E Enhancement of the regenerative capabilities of the neuro- ectomised dog peripheral nerves using nano techniques /

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology

Therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) was reported in several animal models of liver fibrosis. Interleukin (IL) 17A, IL6 and STAT3 have been described to play crucial roles in chronic liver injury. However, the modulatory effect of MSCs on these markers was controversial in different diseases. The immunomodulatory role of BM-MSCs on IL17A/IL6/STAT3 was not fully elucidated in liver fibrosis. In the present study, we evaluated the capacity of the BM-MSCs in the modulation of cytokines milieu and signal transducers, based on unique inflammatory genes Il17a and Il17f and their receptors Il17rc and their effect on the IL6/STAT3 pathway in CCl₄- induced liver fibrosis in rats. A single dose of BM-MSCs was administered to the group with induced liver fibrosis, and the genes and proteins of interest were evaluated along six weeks after treatment. Our results showed a significant downregulation of Il17a, Il17ra, il17f and Il17rc genes. In accordance, BM-MSCs administration declined IL17, IL2 and IL6 serum proteins and downregulated IL17A and IL17RA proteins in liver tissue. Interestingly, BM-MSCs downregulated both Stat3 mRNA expression and p-STAT3, while Stat5a gene was downregulated and p-STAT5 protein was elevated. Also P-SMAD3 and TGFÝR2 proteins were downregulated in response to BM-MSCs treatment. Collectively, we suggest that BM-MSCs might play an immunomodulatory role in the treatment of liver fibrosis through downregulation of IL17A affecting IL6/STAT3 signaling pathway

Issued also as CD

There are no comments on this title.

to post a comment.