Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmaceutical Chemistry

The aim of this work is to develop simple, accurate and precise methods for the determination of some pharmaceutical mixtures in vitro and in human plasma. The drugs were analyzed in their bulk powder and in their pharmaceutical dosage forms either in combinations or in presence combined drugs were determined in plasma. Drugs investigated include Dapagliflozin (SGLT2 inhibitor),Saxagliptin (DPP-4 inhibitor), Metformin (biguanide), Enalapril (ACE-inhibitor), Perindopril (ACEinhibitor), Ramipril (ACE- inhibitor) Atorvastatin calcium and Rosuvastatin calcium (HMG-CoA reductase inhibitor), Ezetimibe (lipid lowering drug), Clopidogrel (adenosine di-phosphate inhibitor) and Telmisartan (angiotensin II blocker) Two LC-MS/MS bioanalytical methods were proposed for the determination of two pharmaceutical mixtures. First mixture is antidiabetic mixture used in treatment of type II diabetes mellitus consisting of dapagliflozin, saxagliptin and metformin. Second mixture consists of three ACE- inhibitors namely, enalapril, perindopril and ramipril. HPLC is a powerful analytical tool for the separation and analysis of pharmaceutical products. It is incorporated in the plan of the work for the estimation of atorvastatin and rosuvastatin in six binary mixtures with other drugs such as ezetimibe, clopidogrel and telmisartan with optimization and application of Box-Behnken experimental design

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