Importance of apoprotein A-I and Sphingosine activity and progression in Egyptian patients : association with a number of long non-coding RNAs expression / Heba Ramadan Ghaiad ; Supervised Amira A. Shaheen , Maha M. Elsawalhi , Mohammed M. Nooh

Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

Several studies pointed out a possible protective role of apoprotein A1 (ApoA1) in inflammation and autoimmunity, besides the involvement of sphingosine 1-phosphate receptors (S1PRs) in mediating such action of ApoA1. However, the exact regulatory mechanisms of these biomolecules and their involvement in multiple sclerosis (MS) remains to be elucidated. This study investigated the role of ApoA1, sphingosine kinase 1 and 2 (SPHK1 & 2), S1PR1 & 5, interferon-Þ (IFN-Þ) and interleukin 17 (IL17) in MS, besides their regulatory long noncoding RNAs (lncRNA): APOA1-AS, IFNG-AS1, and RMRP. Herein, the expression profiles of SPHKs, S1PRs, and lncRNAs were measured in the blood of 72 relapsing-remitting MS (RRMS) patients (37 during relapse and 35 in remission) and 28 healthy controls using qRT-PCR. Plasma levels of ApoA1, IFN-Þ and IL17 were determined. The impact of these parameters was assessed on MS activity, relapse rate, and patient disability. Moreover, the potential use of these parameters as novel diagnostic and prognostic biomarkers for MS was evaluated. Our study demonstrated upregulation of APOA1-AS, IFNG-AS1, SPHK1 & 2, and S1PR5 in RRMS patients. Moreover, significant differences in ApoA1, SPHK2, and IL17 were observed between patients during relapse and in remission. Importantly, ApoA1, SPHK2, and IL17 were related to MS activity, while S1PR1 and IFN-Þ were linked to patient disability, though, only IFN-Þ was associated with relapse rate. Finally, this study provided evidence for excellent diagnostic power of IFN-Þ, IL17, SPHK1 and lncRNA APOA1-AS for MS, whereas SPHK2 showed the highest prognostic power in predicting MS patients in relapse

Issued also as CD