Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

Modified radical mastectomy (MRM) one of conventional treatment of breast cancer was suggested by many evidences that sometimes there is a relation between surgery and tumor recurrence. Most of breast local recurrences occur at the same quadrant of the primary cancer, suggesting that the tumor recurrence may promote from residual cancer cells and/ or quiescent stem cells lasting after surgery in tissues left behind after MRM. CSCs, a subpopulation of cancer cells, are characterized by self-renewal, unlimited and high proliferative potential and apoptosis resistance and the expression of specific cell surface markers. Breast cancer chemo and/or radio resistance, local recurrences and distant metastasis may originate from residual tumor cells and cancer stem cells (CSCs) left behind in the cavity after breast cancer surgery. We hypothesized that the residual malignant cells or quiescent CSCs may be activated by post-surgical tumor microenvironment fluid resulting at the surgical site through factors that are released in response to inflammation. In the present study post-surgical tumor microenvironment fluid were collected within 24 hrs of breast surgery in sterile tubes and filtered. The proteomic composition of post-surgical tumor microenvironment fluid was characterized using RayBio{u2122}human cytokine antibody Post-surgical tumor microenvironment fluid was used to stimulate MDA-MB-231 to test its effect on cell viability by MTT assay. Additionally, expression of CD44/CD24 stem-like surface markers was evaluated for MDA-MB-231 and SUM-149 breast cancer cell lines using Flow Cytometry. Moreover, EMT marker E-cadherin was assessed using immunofluorescence

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