Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

The aim of this study was to evaluate the protective role of the green tea catechins (GTC) against the isoniazid (INH)-induced oxidative stress and associated hematological and biochemical alterations in rats. One hundred male albino rats were divided into four groups: (i) control, (ii) INH-treated, (iii) (INH+GTC)-treated and (iv) GTC-treated groups. In the 2nd and 3rd groups, INH was orally administered at a dose level of 27 mg/kg b.wt., which was equivalent to the therapeutic dose of humans, daily for five weeks. In the 3rd group, GTC were orally administered at a dose level of 50 mg/kg b. wt. one hour prior to INH administration, daily for five weeks. In the 4th group, GTC were orally administered to the animals daily for five weeks at dose level of 50 mg/kg b. wt. From each group, five animals were weekly taken out for sampling of blood and liver.The administration of INH resulted in a normocytic normochromic anemia and leukopenia. Leukopenia was a direct consequence of the reduction in absolute counts of neutrophils, eosinophils, lymphocytes and monocytes. In INH-treated rats, significant increments were observed in serum total cholesterol, LDL-cholesterol, atherosclerotic indices and triglyceride levels whereas HDL-cholesterol level was decreased. Serum total protein and albumin levels were reduced whereas serum globulin level was slightly increased. Serum urea and creatinine levels were not altered in INH-treated rats. Liver function markers (ASAT, ALAT, ALP, total and direct bilirubin) were elevated by the INH administration being indicative to the hepatic damage. Lipid peroxidation was evidenced through the increased level of liver malondialdehyde (MDA) concentrations. The endogenous antioxidant system showed noticeable decrements in liver glutathione (GSH) content and the activities of liver superoxide dismutase (SOD) and catalase (CAT) and erythrocyte glucose-6-phosphate dehydrogenase (G6PD

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