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Influence of laser photobiomodulation and biodentine on proliferation and odontogenic differentiation of human dental pulp mesenchymal stem cells : In-vitro study / Mustafa Najah Hasan Aldarraji ; Supervised Latifa Mohamed Abdelgawad , Dina Sabry Abdelfatah

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mustafa Najah Hasan Aldarraji , 2020Description: 109 P. : charts , facsimiles ; 25cmOther title:
  • تأثير التحفيز الحيوى الضوئى بالليزر مع مادة البايودنتين على الانتشار والتمايز السني للخلايا الجذعية اللبية السنية : دراسة داخل المختبر [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Sciences (NILES) - Department of Laser Application in Oral Surgery Summary: Background: Vital pulp therapy preserves the health of injured dental pulp tissues through improving cells viability and formation of reparative dentine. Objective: Present study was conducted to assess the odontogenic effects of Biodentine{u2122} (BD) and 650nm laser Photobiomodulation (PBM) in dentine regeneration from human dental pulp stem cells (HDPSCs). Methods: Seventy-two HDPSCs well-plates were divided into the following: Group1: control. Group2: treated by BD. Group3: treated by 650nm PBM with 1J/cm2. Group4: treated by both 650nm PBM with 1J/cm2and BD. Group5: treated by 650nm PBM with 2J/cm2. Group6: treated by both 650nm PBM with 2J/cm2and BD. Cell viability was measured by MTT assay for 4days. While odontogenic differentiation of HDPSCs was assessed by alkaline-phosphatase-activity (ALP), Von kossa stain (VKS), and Reverse-transcriptase-PCR (RT-PCR) for dentine-sialophosphoprotein (DSPP), Runt-related-transcription-factor2 (RUNX2) Bone morphogenic protein type 2 (BMP2) and collagen type I (COL I) at day 21. Results: The results showed statistically significant increase in viability of all study groups against control (p-value <0.0001) at 96 hours. Also showed significant differences in ALP activity between groups (p-value <0.0001) and in expression of DSPP, RUNX2, BMP2 and COL I (P<0.001) compared to control. The combination of both BD and PBM 2J/cm2 showed the highest number of calcified nodules
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.24.04.M.Sc.2020.Mu.I (Browse shelf(Opens below)) Not for loan 01010110083018000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.24.04.M.Sc.2020.Mu.I (Browse shelf(Opens below)) 83018.CD Not for loan 01020110083018000
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Cai01.24.04.M.Sc.2020.Ma.E Effect of low power laser irradiation on dental stem cell proliferation and viability in diabetic models : An in vitro study / Cai01.24.04.M.Sc.2020.Mo.E Effect of combined application of growth factors and diode laser bio-stimulation on the osseointegration of dental implants / Cai01.24.04.M.Sc.2020.Mo.E Effect of combined application of growth factors and diode laser bio-stimulation on the osseointegration of dental implants / Cai01.24.04.M.Sc.2020.Mu.I Influence of laser photobiomodulation and biodentine on proliferation and odontogenic differentiation of human dental pulp mesenchymal stem cells : In-vitro study / Cai01.24.04.M.Sc.2020.Mu.I Influence of laser photobiomodulation and biodentine on proliferation and odontogenic differentiation of human dental pulp mesenchymal stem cells : In-vitro study / Cai01.24.04.M.Sc.2020.Ni.A Assessment of The Efficacy of Diode Laser, and Sonic Agitation on Chlorohexidine, and Nanoparticles Irrigants on Bacterial Biofilms in Root Canals / Cai01.24.04.M.Sc.2020.Ni.A Assessment of The Efficacy of Diode Laser, and Sonic Agitation on Chlorohexidine, and Nanoparticles Irrigants on Bacterial Biofilms in Root Canals /

Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Sciences (NILES) - Department of Laser Application in Oral Surgery

Background: Vital pulp therapy preserves the health of injured dental pulp tissues through improving cells viability and formation of reparative dentine. Objective: Present study was conducted to assess the odontogenic effects of Biodentine{u2122} (BD) and 650nm laser Photobiomodulation (PBM) in dentine regeneration from human dental pulp stem cells (HDPSCs). Methods: Seventy-two HDPSCs well-plates were divided into the following: Group1: control. Group2: treated by BD. Group3: treated by 650nm PBM with 1J/cm2. Group4: treated by both 650nm PBM with 1J/cm2and BD. Group5: treated by 650nm PBM with 2J/cm2. Group6: treated by both 650nm PBM with 2J/cm2and BD. Cell viability was measured by MTT assay for 4days. While odontogenic differentiation of HDPSCs was assessed by alkaline-phosphatase-activity (ALP), Von kossa stain (VKS), and Reverse-transcriptase-PCR (RT-PCR) for dentine-sialophosphoprotein (DSPP), Runt-related-transcription-factor2 (RUNX2) Bone morphogenic protein type 2 (BMP2) and collagen type I (COL I) at day 21. Results: The results showed statistically significant increase in viability of all study groups against control (p-value <0.0001) at 96 hours. Also showed significant differences in ALP activity between groups (p-value <0.0001) and in expression of DSPP, RUNX2, BMP2 and COL I (P<0.001) compared to control. The combination of both BD and PBM 2J/cm2 showed the highest number of calcified nodules

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