Assessment of salivary bacterial level and dental caries experience in a group of Egyptian children with black stained teeth : A cross sectional study / Joanna Nassef William Samuel ; Supervised Hany Mohamed Saber , Norhan Abdelwahab Eldokky

Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Pedodontics

Aim: The purpose of this study was to assess the salivary bacterial level and dental caries experience in children with black stained teeth. Methods: Across-sectional study for children aged 4-6 years with black stains where the caries experience was evaluated using dmft index. Also, the salivary sample was taken by into a sterile container and taken to the laboratory within 1 hour for processing. In the laboratory saliva dilution was done and sample was inserted into a plate which contains Brain heart infusion agar. Put the plate into an anaerobic jar and incubated in an incubator. From the plate, subculture identification was done to identify the morphology of Actinomyces. The colony of Actinomyces on the plate was counted with colony counter using colony forming unit method. Results: Among the children with BS, the mean dmft was (2.3), most children were in very low dmft score (42.5%). So this showed lower caries experience in children with black stains in comparison to last national survey. However, the difference was not statistically significant. Regarding the Actinomyces count the results showed higher number of Actinomyces in children with BS. However it

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