Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology

Aim: The aim of our study to investigate and compare the effect of green, medium roasted and decaffeinated coffee on the viability, apoptosis, cell cycle progression, proliferation, invasion and migration of OSCC cell lines. Methodology: Coffee extracts were prepared and oral squamous cell carcinoma cultures were exposed to various concentrations of coffee extracts for 72 hr.Cell viability was assessed and IC50 values for each extract were quantified using MTT assay. Using flowcytometry, apoptotic and necrotic cell population were calculated followed by the assessment of caspase-3 and caspase-9 activity. Cell cycle analysis for each coffee extracts was conducted. Next, EGFRTK assay was done to obtain the inhibition % of tyrosine kinase activity in the studied groups. We assessed the antimigration and anti-invasion effect of coffee extracts on oral squamous cell carcinoma cells using Transwell invasion and migration assay. Finally, HPLC analysis was performed to measure the amount of total phenolic, chlorogenic acid and caffeine in coffee extracts. Results: The results showed a presence of cytotoxic effect of coffee extracts and the IC 50 was 17.1±0.69, 57.95±2.28, and 103.32 ±4.52 in green coffee, roasted coffee and decaffeinated coffee respectively. The total apoptotic and necrotic cell populations was increased in green coffee followed by roasted then decaffeinated compared to control. Capsase-3 and caspase-9 activity was up-regulated in all coffee extract groups with the same order. All coffee extracts arrested cell cycle at G2-M phase

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