Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Veterinary Pharmacology

The present study assessed the long-term daily administration of Titanium dioxide "TiO₂, E1713 & Acrylamide (ACR). Titanium dioxide and acrylamide are widely used as a food additives that exists in various everyday food products all over the world together with vast applications in cosmetics and industry. However, several toxicological issues, particularly after oral exposure. This study was planned to investigate the effect of oral exposure through oral gavage once daily for 90 consecutive days to two doses of TiO₂ and ACR which are (20 or 40 mg/kg bwt) AND (1 or 2 mg/kg b.wt.) respectively on the blood components, immunity, cytotoxic, and genotoxic indicators. The results revealed that a dose-dependent make leukopenia, eosinophilia, neutrophilia, and thrombocytopenia were noted. Also, the immunoglobins G (IgG) and IgM were significantly elevated the lysozyme levels, nitric oxide concentration, and phagocytic activity, immunoglobulin levels were significantly depleted. A significant reduced in lymphocyte proliferation but elevated LDH activity was prominent finally a marked increase in CD4 and CD8 immunolabeling was evident. Overall, these results indicated that TiO₂ and ACR exert hepatotoxic and immunotoxic effects with long-term exposure. these results indicated that the two-food additives could induce hematotoxicity, genotoxic, and immunotoxic alterations with exposure for long durations

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