Preparation of inactivated lumpy skin disease virus vaccine using ISA 206 and ISA 50 adjuvants / Youssef Abdallah Elsayed AbdElhafiz ; Supervised Mohammed Abdelhameed Shalaby , Ayman Hany M. Eldeeb , Mervat Mohamed Ali

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

Lumpy skin disease (LSD) is one of most serious livestok diseases in Egypt since 1988 when the disease spread widely all over the country. since that attenuated sheep pox virus (SPV) used for vaccination cattle flocks against LSD due to immunological relationship between LSD and SPV. There have been numerous attempts to produce live attenuated LSD vaccine but it has stopped due to the seriousness of the disease's return to its virulence and the post vaccinal reaction of the vaccine so we prepare inactivated LSD virus(LSDV) vaccine to immunize the cattle with safe vaccine .Ismailia isolate of LSD was propagated and titrated on Madin Darby Bovine Kidney Cells (MDBK) till reach the highest titer (10 ⁵·⁵ TCID₅₀/ml). The inactivation process was performed using Bromoethylamine hydro bromide (BEI) that did not affect the immunogenic characters of the LSDV and the process last for 18 hours with 1% concentration of BEI at 37{u00B0}c. The inactivated LSDV was tested on MDBK cells and the animals free of antibodies for residual live virus and determination of minimal protective dose (MPD) of prepared inactivated LSDV. The MPD was 1.5 ml of the inactivated virus and the vaccine was prepared by adding equal volume of the adjuvants (Montanide ISA 50 and Montanide ISA 206) to the total quantity 3ml of the vaccine

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