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Increased integrity of free circulating DNA in sera of patients with colorectal cancer / Reem Hasanin Mohamed Ali ; Supervised Dina Farouk Elgyaar , Nevine Ezz Eldin Elabd , Noha Hassan Ibrahim

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Reem Hasanin Mohamed Ali , 2015Description: 147 P. : charts ; 25cmOther title:
  • دراسة سلامة الحمض النووى الحر كعلامة بيولوجية مصلية محتملة للكشف عن سرطان القولون و المستقيم [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Cell - free DNA circulating in blood is a candidate biomarker for malignant tumors. Unlike uniformly truncated DNA released from apoptotic non diseased cells, DNA released from dead cancer cells varies in size. To measure the ratio of longer to shorter DNA fragments (DNA integrity) in serum to study it as a potential biomarker for patients with colorectal cancer (CRC). Sera from 50 patients with CRC,10 patients with benign colonic lesions and 20 healthy volunteers were assessed by quantitative real-time PCR of ALU repeats (ALU-qPCR) with 2 sets of primers (115 and 247 bp) amplifying different lengths of DNA. DNA integrity was determined as ratio of qPCR results of ALU 247 - bp over ALU 115-bp. Serum DNA integrity was increased in cases of CRC at different stages indicating highly significant difference from control and premalignant groups (p < 0.001). ROC curves for differentiating CRC from control and from premalignant lesions had areas under the curves of 0.9 and 0.85 respectively
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2015.Re.I (Browse shelf(Opens below)) Not for loan 01010110066951000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2015.Re.I (Browse shelf(Opens below)) 66951.CD Not for loan 01020110066951000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Cell - free DNA circulating in blood is a candidate biomarker for malignant tumors. Unlike uniformly truncated DNA released from apoptotic non diseased cells, DNA released from dead cancer cells varies in size. To measure the ratio of longer to shorter DNA fragments (DNA integrity) in serum to study it as a potential biomarker for patients with colorectal cancer (CRC). Sera from 50 patients with CRC,10 patients with benign colonic lesions and 20 healthy volunteers were assessed by quantitative real-time PCR of ALU repeats (ALU-qPCR) with 2 sets of primers (115 and 247 bp) amplifying different lengths of DNA. DNA integrity was determined as ratio of qPCR results of ALU 247 - bp over ALU 115-bp. Serum DNA integrity was increased in cases of CRC at different stages indicating highly significant difference from control and premalignant groups (p < 0.001). ROC curves for differentiating CRC from control and from premalignant lesions had areas under the curves of 0.9 and 0.85 respectively

Issued also as CD

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